Pre-exposure to heat shock inhibits peroxynitrite-induced activation of poly(ADP) ribosyltransferase and protects against peroxynitrite cytotoxicity in J774 macrophages

doi:10.1016/S0014-2999(96)00628-0

European Journal of Pharmacology

Volume 315, Issue 2, 14 November 1996, Pages 221-226

https://doi.org/10.1016/S0014-2999(96)00628-0 Get rights and content

Abstract

The reaction of nitric oxide (NO) with superoxide yields the cytotoxic oxidant peroxynitrite, produced during inflammation and shock. A novel pathway of peroxynitrite cytotoxicity involves activation of the nuclear enzyme poly(ADP) ribosyltransferase, and concomitant ADP-ribosylation, NAD⁺ consumption and exhaustion of intracellular energy stores. In the present report we provide evidence that pre-exposure of J774 macrophages to heat shock reduces peroxynitrite-induced activation of poly(ADP) ribosyltransferase and protects against the peroxynitrite-induced suppression of mitochondrial respiration. The protection was significant at 8 h after heat shock, but was absent at 24 h after heat shock. Thus, the protection showed a temporal correlation with the expression of heat shock protein 70, the expression of which was maximal at 8 h. Exposure to heat shock did not alter the expression of poly(ADP) ribosyltransferase over 24 h. In summary, the heat shock phenotype or heat shock proteins may protect against peroxynitrite induced cytotoxicity.

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Pre-exposure to heat shock inhibits peroxynitrite-induced activation of poly(ADP) ribosyltransferase and protects against peroxynitrite cytotoxicity in J774 macrophages

Abstract

Arch. Biochem. Biophys.

The comparative toxicity of nitric oxide and peroxynitrite to Escherichia coli

FEBS Lett.

J. Biol. Chem.

J. Biol. Chem.

FEBS Lett.

Biochem. Biophys. Res. Commun.

FEBS Lett.

Gastroenterology

Arch. Biochem. Biophys.

Arch. Biochem. Biophys.

Chem.-Biol. Interact.

Biochem. Biophys. Res. Commun.

FEBS Lett.

FEBS Lett.

Heat shock induces resistance in rat pancreatic islet cells against nitric oxide, oxygen radicals and streptozotocin toxicity in vitro

J. Clin. Invest.

Nitric oxide: role in neurotoxicity

Clin. Exp. Pharmacol. Physiol.

Autocrine inhibition of Na + /K(+)-ATPase by nitric oxide in mouse proximal tubule epithelial cells

J. Clin. Invest.

Peroxynitrite inhibition of oxygen consumption and sodium transport in alveolar type II cells

Am. J. Physiol.