Effect of storage at — 70°C on lipid, lipoprotein and apolipoprotein concentrations
References (20)
- et al.
Suitability of frozen and lyophilized sera for cholesterol and triglyceride determinations
Clin Chim Acta
(1982) - et al.
Effect of storage on plasma lipoproteins (letter)
Lancet
(1977) - et al.
Determination of high density lipoprotein-cholesterol in stored human plasma
J Lipid Res
(1980) - et al.
Precautionary measures for collecting blood destined for lipoprotein isolation
Methods Enzymol
(1986) - et al.
Sequential flotation ultracentrifugation
Methods Enzymol
(1986) - et al.
A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples
Anal Biochem
(1978) - et al.
Stability of total cholesterol, high-density lipoprotein cholesterol and triglycerides in frozen sera
Clin Chem
(1986) - et al.
High-density lipoprotein quantitation: effect of plasma storage on heparin-Mn2 + supernatant cholesterol concentration
Clin Chem
(1979) - et al.
High-density lipoprotein subfractions measured in stored serum
Clin Chem
(1994) - et al.
Evaluation of long-term frozen storage of plasma for measurement of high-density lipoprotein and its subfractions by precipitation
Clin Chem
(1990)
Cited by (19)
HDL structure and function is profoundly affected when stored frozen in the absence of cryoprotectants
2017, Journal of Lipid ResearchCitation Excerpt :Thus, long-term storage of plasma at −20°C does not seem to be appropriate for subsequent quantification of HDL-cholesterol and its subclasses after precipitation (25). Storage at −70°C was preferable, but also led to a decrease in HDL protein content (36), which is in good agreement with our results from structural analysis. In a recent study, the impact of storage at −20°C on the distribution of apoC-III and apoE within HDL was investigated (37).
Study of frozen low density lipoprotein particles by using nanotechnology
2015, Clinical BiochemistryCitation Excerpt :This notion has been supported by a few analytical studies investigating the impact of storage and handling of blood plasma on the accuracy of analysis. Several studies showed an increase in lipid concentrations by freezing [1,2], whereas others showed a decrease [3,4], or no significant alterations in lipid content [5]. Moreover, a later study of Zivkovic et al. [6] has shown that a single freeze–thaw cycle resulted in a variability of about 37% in HDL- and LDL-cholesterol.
Increased endoplasmic reticulum stress and Nrf2 repression in peripheral blood mononuclear cells of patients with stable coronary artery disease
2014, Free Radical Biology and MedicineCitation Excerpt :C-reactive protein (CRP) was measured using a commercially available high-sensitivity turbidimetric method (Syncron-PCR, Beckman Coulter, Brea, CA, USA). Plasma for LDL separation was frozen and stored as previously reported [18]. Samples were kept frozen for no longer than 12 months, with an average of 7 months, and thawed only once.
Impact of freezing on high-density lipoprotein functionality
2008, Analytical Biochemistry