Circadian rhythms in mouse suprachiasmatic nucleus explants on multimicroelectrode plates

doi:10.1016/S0006-8993(97)00337-5

Brain Research

Volume 757, Issue 2, 23 May 1997, Pages 285-290

https://doi.org/10.1016/S0006-8993(97)00337-5 Get rights and content

Abstract

The suprachiasmatic nucleus (SCN) of the mammalian hypothalamus functions as a circadian pacemaker. This study used multimicroelectrode plates to measure extracellular action potential activity simultaneously from multiple sites within the cultured mouse SCN. Neurons within the isolated mouse SCN expressed a circadian rhythm in spontaneous firing rate for weeks in culture.

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These now-classic studies are summarized in the published report of a meeting held to evaluate the state of SCN research on the 25th anniversary of its discovery.3 Studies using a variety of in vitro models, including electrophysiological recording and optical monitoring of SCN cell and tissue cultures, have provided compelling evidence that circadian oscillation is fundamentally a cell-autonomous process, expressed in many, but probably not all, individual SCN neurons.4–7 Nevertheless, individual SCN clock cells normally interact to produce coherent circadian signals at the tissue (and behavioral) level.8–10
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¹: Current address: Brigham and Women's Hospital/Harvard Medical School/Boston, MA 02115.

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Short communicationCircadian rhythms in mouse suprachiasmatic nucleus explants on multimicroelectrode plates

Abstract

Neuroscience

Brain Res.

Neuron

J. Theor. Biol.

Brain Res.

Brain Res.

Brain Res.

Physiol. Behav.

Brain Res.

Neurosci. Lett.

Neurosci. Lett.

Methods Enzymol.

J. Neurosci. Methods

Brain Res. Bull.

Brain Res.

Brain Res.

Brain Res.

Brain Res.

Brain Res.

Neuron

Brain Res.

Multielectrode analysis of coordinated, multisite, rhythmic bursting in cultured CNS monolayer networks

J. Neurosci.

Simultaneous single unit recording in vitro with a photoetched laser deinsulated gold multimicroelectrode surface

IEEE Trans. Biomed. Eng.

Short communication
Circadian rhythms in mouse suprachiasmatic nucleus explants on multimicroelectrode plates