4.14 - Insect Cell Culture and Recombinant Protein Expression Systems
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Cited by (10)
Stably Transformed Insect Cell Lines: Tools for Expression of Secreted and Membrane-anchored Proteins and High-throughput Screening Platforms for Drug and Insecticide Discovery
2006, Advances in Virus ResearchCitation Excerpt :Insect cell‐based expression systems for continuous production of recombinant proteins show certain distinct features that make them suitable for a growing number of applications. These features (summarized in Farrell et al., 2005) include fast process from cDNA cloning to protein production (stable cell lines are developed within 1–2 months), correct intron splicing for expression from genomic DNA, full capacity of posttranslational modifications (since integrity of the cells is maintained throughout the production process), continuity of protein production (the transgene is stably integrated into the genome and proteins are produced continuously), limited proteolysis due to absence of cell lysis (because no virus infection occurs), stable physiological environment for membrane protein expression, high yields of secreted recombinant proteins, and easy purification of secreted proteins from serum‐free media. On the other hand, possible limitations of this system may lie in the correct folding, and the extent and type of posttranslational modifications (especially glycosylation; see chapter by Harrison and Jarvis, this volume, pp. 159–191) taking place in insect cells, in order to obtain production of bioactive proteins (i.e., with regard to the need for “humanized” glycoprotein production for the pharmaceutical industry).
Pulsed ultrasound for enhancing vaccine production
2015, Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBSInsect viruses
2013, Fields Virology: Sixth EditionApplication of baculovirus-insect cell expression system for human therapy
2011, Current Pharmaceutical Biotechnology