Overexpression, one-step purification and characterization of UDP-glucose dehydrogenase and UDP-N-acetylglucosamine pyrophosphorylase
The two enzymes were overexpressed and used in the synthesis of Hyaluronic Acid.
References (29)
- et al.
J. Biol. Chem.
(1959) - et al.
J. Biol. Chem.
(1993) - et al.
J. Biol. Chem.
(1993) - et al.
J. Biol. Chem.
(1959) - et al.
Biochim. Biophys. Acta
(1976) - et al.
J. Org. Chem.
(1982) - et al.
J. Am. Chem. Soc.
(1991) - et al.
J. Am. Chem. Soc.
(1992) - et al.
Angew. Chem. Int. Ed. Engl.
(1995) Science
(1971)
Biochem. J.
J. Bacteriol.
Nature
Cited by (13)
Enzyme cascades for the synthesis of nucleotide sugars: Updates to recent production strategies
2023, Carbohydrate ResearchCitation Excerpt :In the past years, the salvage pathways, in particular, have become the more frequently used enzyme module systems for the in vitro production of nucleotide sugars, as their simplicity makes them easier to optimize for large-scale production of nucleotide sugars, as outlined in the following chapters. The de novo synthesis of UDP-Glc is typically catalyzed by an enzyme cascade, including hexokinase (EC 2.7.1.1) or glucokinase (EC 2.7.1.2), phosphoglucomutase (EC 5.4.2.2), and UDP-glucose pyrophosphorylase (EC 2.7.7.9) [30,49–53]. However, the synthesis of UDP-Glc made a significant step toward productivity using sucrose synthase known from plants and bacteria [34,54] (Table S1).
Cloning, expression and biochemical characterization of UDP-glucose 6-dehydrogenase, a key enzyme in the biosynthesis of an anti-tumor polysaccharide from the marine fungus Phoma herbarum YS4108
2011, Process BiochemistryCitation Excerpt :UDP-GlcA has also been shown to be a common precursor for nucleotide sugars including UDP-galacturonic acid, UDP-arabinose, UDP-xylose and UDP-apiose, which are essential for the biosynthesis of hemicellulose and pectin [2]. In mammals, UDP-GlcDHase provides precursors for an array of extracellular matrix glycosaminoglycans such as heparin and hyaluronic acid, and also catalyzes the crucial structural modification of xenobiotic toxins required for their eventual eliminations [3]. In microorganisms, the UDP-GlcDHase gene has been shown to be necessary for the biosynthesis of many virulence-associated exopolysaccharides [4–7], as well as of glycans such as xanthan gum [4,5] and gellan gum [8–10], which are of high industrial and/or medicinal values.
Enzymatic Approaches to O-Glycoside Introduction: Glycosyltransferases
2007, Comprehensive Glycoscience: From Chemistry to Systems BiologyIdentification of novel inhibitors of UDP-Glc 4′-epimerase, a validated drug target for african sleeping sickness
2006, Bioorganic and Medicinal Chemistry LettersProperties and kinetic analysis of UDP-glucose dehydrogenase from group A streptococci. Irreversible inhibition by UDP-chloroacetol
1997, Journal of Biological ChemistryEfficient chemoenzymatic synthesis of uridine 5′-diphosphate N-acetylglucosamine and uridine 5′-diphosphate N-trifluoacetyl glucosamine with three recombinant enzymes
2017, Preparative Biochemistry and Biotechnology