Advances in the use of Pichia pastoris for high-level gene expression
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Screening and efficient production of engineered lipase B from Candida Antarctica for eco-friendly recycling of waste polycaprolactone
2022, Polymer Degradation and StabilityPichia pastoris Protein Disulfide Isomerase (PDI1) promoter for heterologous protein production and its sequence characterization
2020, Enzyme and Microbial TechnologypMOX: a new powerful promoter for recombinant protein production in yeast Pichia pastoris
2020, Enzyme and Microbial TechnologyCloning, expression, and characterization of a porcine pancreatic α-amylase in Pichia pastoris
2018, Animal NutritionCitation Excerpt :Interestingly, our method was effective as the a-factor signal peptide in the yeast expression vector guided the secretion of the recombined rePPA into the culture broth. This approach can overcome the complicated purification procedures and can be applied for a direct industrial application for amylase (Romanos, 1995). The enzyme yield (65 mg/L) by the methanol-inducible Pichia expression system was higher than that of Rhizopus oryzae α-amylase (20 mg/L) produced in Kluyveromyces lactis (Li et al., 2011), but lower than that of mouse salivary α-amylase (240 mg/L) expressed in P. pastoris (Kato et al., 2001).
New insight into the method of posttransformational vector amplification (PTVA) in Pichia pastoris
2018, Journal of Microbiological MethodsHeterologous expression and biochemical characterization of a novel thermostable Sclerotinia sclerotiorum GH45 endoglucanase in Pichia pastoris
2018, International Journal of Biological Macromolecules
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