Promotion of germination and shoot elongation of some plants by alginate oligomers prepared with bacterial alginate lyase

doi:10.1016/0922-338X(93)90181-7

Journal of Fermentation and Bioengineering

Volume 75, Issue 1, 1993, Pages 68-70

Journal of Fermentat...

https://doi.org/10.1016/0922-338X(93)90181-7 Get rights and content

Abstract

Depolymerization of sodium alginate (average molecular weight: 25,700) from an edible seaweed Eisenia bicyclis with bacterial alginate lyase yielded oligosaccharide(s) with an average molecular weight of 1,800 together with other components. The proliferation and/or differentiation of plants was markedly enhanced in the presence of the oligosaccharide(s), although that of mammalian (HeLa) cells and an algae (Chlamidomonas sp.) was not. The results indicate that the depolymerization products of alginate containing oligosaccharine-like compounds specifically affect the proliferation and/or differentiation of higher plants.

References (19)

J.D. Anderson et al.
Induction of ethylene biosynthesis in tobacco leaf discs by cell wall digesting enzymes
Biochem. Biophys. Res. Commun.
(1982)
P.D. Bishop et al.
Isolation and characterization of the proteinase inhibitor-inducing factor from tomato leaves
J. Biol. Chem.
(1984)
Y. Yonemoto et al.
Bacterial alginate lyase: characterization of alginate lyase-producing bacteria and purification of the enzyme
J. Ferment. Bioeng.
(1991)
K. Murata et al.
Continuous depolymerization of alginate by a non-support bioreactor system containing flocculated bacterial cells
J. Ferment. Bioeng.
(1992)
Y. Yonemoto et al.
Cloning of a gene for intracellular alginate lyase in a bacterium isolated from a ditch
J. Ferment. Bioeng.
(1992)
O.H. Lowry et al.
Protein measurement with Folin-phenol reagent
J. Biol. Chem.
(1951)
P. Albersheim et al.
Oligosaccharides
Sci. Am.
(1985)
M.G. Harn et al.
Host-pathogen interactions
Plant Physiol.
(1981)
K. Tran Thanh Van et al.
Manipulation of the morphogenetic pathways of tobacco explants by oligosaccharides
Nature (London)
(1985)

There are more references available in the full text version of this article.

Cited by (98)

Exogenous application of coronatine and alginate oligosaccharide to maize seedlings enhanced drought tolerance at seedling and reproductive stages
2023, Agricultural Water Management
Plant growth regulators (PGRs) play crucial roles in maize drought tolerance. However, most of the studies are focused on seedling stage under greenhouse conditions, little is known on the effects of grain yields at later growth stage under field environment. It is hypothesized that exogenous application of PGRs can increase maize drought tolerance at different growth stages under field conditions. Here we used alginate oligosaccharide (AOS) and coronatine (COR) to investigate the effects of these two PGRs on maize drought tolerance in a two-year field experiment. The results showed that both COR and AOS treatments reduced leaf water loss and significantly increased the relative leaf water content by 12.6% and 13.0%, thus significantly decreasing superoxide anion accumulation by 49% and 42% in maize seedlings. There was no big difference between these two treatments in the seedling stage. In the subsequent reproductive growth period, although both COR and AOS treatments significantly increased leaf photosynthesis and above-ground growth, including photosynthetic rate by 41% and 33%, relative chlorophyll content (SPAD value) by 8.0% and 6.5%, leaf area by 22% and 15%, plant height by 8.3% and 5.9%, dry matter accumulation by 23% and 17%, respectively, under drought conditions, the effect of COR was superior to the AOS, as evidenced by membership function values of COR treatment were 0.12 and 0.08 larger than that of AOS treatment in 2020 and 2021, respectively. Ultimately, COR and AOS treatments significantly increased the grain yields by 21.7% and 16.2% in 2020, 22.3% and 15.7% in 2021, respectively, under drought conditions. Path analysis showed both PGRs increased final grain yields by increasing thousand-kernel weight (TKW). While the TKW increase with COR treatment was mainly due to the increase of relative chlorophyll content (SPAD value), AOS treatment mainly increased dry matter accumulation under drought conditions.
Exploring a library of water-soluble polymers as abiotic phytoremediation agents for treating (Pseudo)metal ion-contaminated soil
2022, Chemosphere
Herein, we investigate the effect of eleven water-soluble polymers on the growth of Sedum alfredii and its uptake of As, Cd, or Pb in polluted soil in a pot experiment. The polymers selected are generally non-toxic, metal-chelating, and hygroscopic. They also range from acidic (carboxylate-containing polymers) to neutral (e.g. polyacrylamide, polyvinyl alcohol) and to basic (polyethylene imine). It has been found that sodium alginate exhibits a significant growth-promoting effect (as much as ∼8-fold) as determined by plant total dry weight, while all other tested polymers exert either minor growth promotion or a negative effect. To examine the absorption of heavy (pseudo)metals, the pollutant content in above- and below-ground portions of the plant were separately studied by inductively coupled plasma mass spectrometry. Among the tested polymers, sodium alginate emerges as the strongest absorption accelerator for all three metal pollutants in the above-ground part of the plant (∼3-4-fold increase over blank), mainly due to promotion of plant growth. On the other hand, polyethylene imine is the most potent inhibitor of metal absorption (10%–51% of blank) due to a combination of plant growth inhibition and absorption deactivation. Polymers with such effects may be used to reduce the contamination of (pseudo)metals in crops.
Chitosan-, alginate- carrageenan-derived oligosaccharides stimulate defense against biotic and abiotic stresses, and growth in plants: A historical perspective
2021, Carbohydrate Research
During the last 20 years, the mechanisms involved in the stimulation of defense against pathogens, and growth triggered by chitosan-, alginate- and carrageenan-derived oligosaccharides have been studied in plants. Oligo-chitosan stimulate protection against pathogens by activation of salicylic acid (SA) or jasmonic acid/ethylene (JA/ET)-dependent pathways, protection against abiotic stress through abscisic acid (ABA)-dependent pathway, and growth by increasing photosynthesis, auxin and gibberellin content, C and N assimilation, and synthesis of secondary metabolites with antipathogenic and medicinal properties. Oligo-alginates stimulate protection against pathogens through SA-dependent pathway, abiotic stress via ABA-dependent pathway, and growth by increasing photosynthesis, auxin and gibberellins contents, C and N assimilation, and synthesis of secondary metabolites with antipathogenic and medicinal properties. Oligo-carrageenan increased protection against pathogens through JA/ET, SA- and Target of Rapamycin (TOR)-dependent pathways, and growth by activation of TOR-dependent pathway leading to an increase in expression of genes involved in photosynthesis, C, N, S assimilation, and enzymes that synthesize phenolic compounds and terpenes having antipathogenic activities. Thus, the latter oligosaccharides induce similar biological effects, but through different signaling pathways in plants.
Effect of presowing treatment of Co-60 gamma-irradiated chitosan on seed germination and seedling vigor in Eucalyptus citriodora Hook
2021, Radiation-Processed Polysaccharides: Emerging Roles in Agriculture
Eucalyptus citriodora Hook. is extremely valued for its essential oil. The establishment of plantations of Eucalyptus from seedlings relies on successful seed germination. Since germination could be vital stage within the life cycle of plants. Application of oligomers of low molecular mass on plants brings considerable numerous changes in biological and physiological activities, as well as plant growth. Irradiated chitosan (ICN) is taken into account to act as signal molecules of plant through regulation of genes. The aim of this experiment was to find out the best soaking concentration of different aqueous solution of ICN on germination parameters of E. citriodora. The seeds were treated with aqueous solution of different concentrations of un-irradiated chitosan (UCN) at 30 mg L^− 1 and four different aqueous concentrations of ICN (irradiated at 250 KGy) viz. 30, 60, 90 and 120 mg L^− 1 and double distilled water (DDW) served as controls. The effect of ICN was found significant for most of the parameters. The concentration of ICN at 60 mg L^− 1 proved optimum for the overall development of Eucalyptus.
Preparation of low-molecular-weight sodium alginate by ozonation
2021, Carbohydrate Polymers
Low-molecular-weight sodium alginate (LMWSA) has been reported to possess unique physicochemical properties and bioactivities. There is little information available about degradation of sodium alginate by ozonation. Effect of ozonation on molecular weight, molecular weight distribution, color change, M/G ratio, and chemical structure of sodium alginate was investigated. The molecular weight of sodium alginate decreased from 972.3 to 76.7 kDa in the 80-min period of ozonation at 25 °C. Two different degradation-rate constants were calculated. Molecular weight distribution of the LMWSA changed appreciably. Ozonation cannot lead to color change of LMWSA. The M/G ratio of LMWSA was not altered significantly, compared with that of the original alginate. The FT-IR and ¹³C NMR spectra indicated the chemical structure of LMWSA obtained by ozonation was not altered appreciably. New insight into the ozonation of alginate will be promisingly opened up. Ozonation of sodium alginate can be a alternative for production of LMWSA.
Preparation of alginate oligosaccharides and their biological activities in plants: A review
2020, Carbohydrate Research
Citation Excerpt :
As both the composition and Mw could affect the biological activity of AOS, we therefore classified previous reports according to the different structures and compositions of AOS as they relate to promotion of plant growth at different stages (As shown as Table 1). In 1992, Yonemoto et al. reported a bacterial alginate lyase from an edible seaweed Eisenia bicyclis and used it to degrade alginate (average Mw: 25.7 kDa) to oligosaccharides (average Mw: 1.80 kDa) [74]. For the first time, it was found that AOS specifically promoted the proliferation and differentiation of higher plants, and that rice germination was also improved [75].
Alginate oligosaccharide (AOS) is the degradation product of alginates extracted from brown algae. As a multifunctional oligomer, it has attracted widespread attention in plant research. Different methods of preparation generate AOS possessing diverse structural properties, and result in differences in AOS activity. In this review, the methods of preparation and characterization of AOS are briefly summarized, followed by a systematic introduction to the activity and mechanisms of AOS in plants. AOS can act as a growth promoter at different growth stages of plants. AOS also enhances resistance to pathogens, drought, salt, heavy metals and other stressors by triggering plant immunity, exerting bioactivity just like a pathogen-associated molecular pattern. In addition, AOS can regulate ABA biosynthesis and metabolite to preserve fruit quality and enhance shelf life. This review provides a comprehensive summary of the biological activity of AOS in plants, which will support research and the application of AOS treatments for plants in the future.

View all citing articles on Scopus

View full text

NotePromotion of germination and shoot elongation of some plants by alginate oligomers prepared with bacterial alginate lyase

Abstract

Biochem. Biophys. Res. Commun.

J. Biol. Chem.

J. Ferment. Bioeng.

J. Ferment. Bioeng.

J. Ferment. Bioeng.

J. Biol. Chem.

Oligosaccharides

Sci. Am.

Host-pathogen interactions

Plant Physiol.

Manipulation of the morphogenetic pathways of tobacco explants by oligosaccharides

Nature (London)

Note
Promotion of germination and shoot elongation of some plants by alginate oligomers prepared with bacterial alginate lyase