Neuron
Volume 2, Issue 4, April 1989, Pages 1313-1323
Journal home page for Neuron

Article
Molecular characterization of the drosophila trp locus: A putative integral membrane protein required for phototransduction

https://doi.org/10.1016/0896-6273(89)90069-XGet rights and content

Abstract

Recent studies suggest that the fly uses the inositol lipid signaling system for visual excitation and that the Drosophila transient receptor potential (trp) mutation disrupts this process subsequent to the production of IP3. In this paper, we show that trp encodes a novel 1275 amino acid protein with eight putative transmembrane segments. Immunolocalization indicates that the trp protein is expressed predominantly in the rhabdomeric membranes of the photoreceptor cells.

References (63)

  • J.E. O'Tousa et al.

    The Drosophila ninaE gene encodes an opsin

    Cell

    (1985)
  • M. Schiffer et al.

    Use of helical wheels to represent the structures of proteins and to identify segments with helical potential

    Biophys. J.

    (1967)
  • S. Supattapone et al.

    Solubilization, purification, and characterization of an inositol trisphosphate receptor

    J. Biol. Chem.

    (1988)
  • D.M. Watterson et al.

    The complete amino acid sequence of the Ca2+-dependent modulator protein (calmodulin) of bovine brain

    J. Biol. Chem.

    (1980)
  • C.S. Zuker et al.

    Isolation and structure of a rhodopsin gene from D. melanogaster

    Cell

    (1985)
  • S. Barash et al.

    Light reduces the excitation efficiency in the nss mutant of the sheep blowfly Lucilia

    J. Gen. Physiol.

    (1988)
  • M.J. Berridge

    Inositol trisphosphate and diacylglycerol: two interacting second messengers

    Annu. Rev. Biochem.

    (1987)
  • M.D. Biggin et al.

    Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination

  • A. Blumenfeld et al.

    Lihht-activated guanosinetriphosphatase in Musca eye membranes resembles the prolonged depolarizing afterpotential in photoreceptor cells

  • P. Brandt et al.

    A C-terminal, calmodulin-like regulatory domain from the plasma membrane Ca2+-pumping ATPase

  • W.A. Catterall

    Structure and function of voltage-sensitive ion channels

    Science

    (1988)
  • D.J. Cosens et al.

    Abnormal electroretinogram from a Drosophila mutant

    Nature

    (1969)
  • A.F. Cowman et al.

    An opsin gene expressed in only one photoreceptor cell type of the Drosophila eye

    Cell

    (1985)
  • L. Dente et al.

    pEMBL: a new family of single stranded plasmids

    Nucl. Acids Res.

    (1983)
  • O. Devary et al.

    Coupling of photoexcited rhodopsin to phosphoinositide hydrolysis in fly photoreceptors

  • K.J. Fryxell et al.

    An opsin gene that is expressed only in the R7 photoreceptor cell of Drosophila

    EMBO J.

    (1987)
  • B. Ganetzky et al.

    Neurogenetics of membrane excitability in Drosophila

    Annu. Rev. Genet.

    (1986)
  • A.F. Gilman

    G proteins: transducers of receptor-generated signals

    Annu. Rev. Biochem.

    (1987)
  • J.C. Hall

    Genetics of the nervous system in Drosophila

    Quart. Rev. Biophys.

    (1982)
  • T.D. Hill et al.

    Inositol 1,3,4,5tetrakisphosphate induces Ca2+ sequestration in rat liver cells

    Science

    (1988)
  • H. Inoue et al.

    Membrane-associated phosopholipase C of Drosophila retina

    J. Biochem.

    (1988)
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    Present address: Department of Biological Chemistry and Department of Neuroscience, The Johns Hopkins University, School of Medicine, 725 N. Wolfe Street, Baltimore, Maryland 21205.

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