Resistance of wheat to Puccinia graminis f.sp. tritici: Association of the hypersensitive reaction with the cellular accumulation of lignin-like material and callose

doi:10.1016/0885-5765(90)90100-C

Physiological and Molecular Plant Pathology

Volume 36, Issue 2, February 1990, Pages 109-120

https://doi.org/10.1016/0885-5765(90)90100-C Get rights and content

Abstract

Seedlings of wheat (Triticum aestivum L.) containing the Sr5 gene for resistance to wheat stem rust were inoculated with race 32 of Puccinia graminis (Pers.) f.sp. tritici Eriks. & E. Henn., which contains the corresponding P5 gene for avirulence, and primary leaves were examined microscopically for host-parasite interaction. Infected epidermal and mesophyll cells containing the Sr5 gene reacted hypersensitively, with accumulation of compounds that autofluoresce yellow during excitation with ultraviolet light. Histochemical tests for lignin using phloroglucinol and chlorinesulphite were positive in hypersensitively necrotic wheat cells. In addition, callose was localized in yellow fluorescing necrotic cells by treatment with resorcinol blue or lacmoid. Products of radiolabelled [¹⁴C]-cinnamic acid accumulated in the hypersensitively necrotic wheat cells. In vivo application of the competitive inhibitor of phenylalanine ammonia-lyase (PAL, E.C. 4.3.1.5), α-aminooxyacetate, inhibited the hypersensitive reaction and markedly altered resistance expression. The data provide evidence that the accumulation of lignin or lignin-like material is associated with the hypersensitive reaction conditioned by the Sr5 gene.

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^☆: The work was supported by the Deutsche Forschungsgemeinschaft.

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Resistance of wheat to Puccinia graminis f.sp. tritici: Association of the hypersensitive reaction with the cellular accumulation of lignin-like material and callose☆

Abstract

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The estimation of relative intracellular phenylalanine ammonia-lyase (PAL)-activities and the modulation in vivo and in vitro by competitive inhibitors

Berichte der Deutschen Botanischen Gesellschaft

Cellular lignification as a factor in the hypersensitive resistance of wheat to stem rust

Physiological Plant Pathology

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Annual Review of Plant Physiology

A light and scanning-electron microscopic study of infections of tomato plants by virulent and avirulent races of Cladosporium fulvum

Netherlands Journal of Plant Pathology

Metabolism of aromatic compounds in healthy and rust-infected primary leaves of wheat. II. Studies with L-phenylalanine-U-14C, L-tyrosine-U-14C, and ferulate-U-14C

Canadian Journal of Botany

Electron microscopy of susceptible and resistant near-isogenic (sr6Sr6) lines of wheat infected by Puccinia graminis tritici. III. Ultrastructure of incompatible interactions

Canadian Journal of Botany

Modification of L-phenylalanine ammonia-lyase in soybean cell suspension cultures by 2-aminooxyacetate and L-2-aminooxy-3-phenylpropionate

Planta

Metabolism of aromatic compounds in healthy and rust-infected primary leaves of wheat. II. Studies with L-phenylalanine-U-¹⁴C, L-tyrosine-U-¹⁴C, and ferulate-U-¹⁴C

Electron microscopy of susceptible and resistant near-isogenic ( $sr6 Sr6$ ) lines of wheat infected by Puccinia graminis tritici. III. Ultrastructure of incompatible interactions