Purification and characterization of a host-selective necrosis toxin from Pyrenophora tritici-repentis

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Abstract

Isolates of Pyrenophora tritici-repentis, the causal agent of tan spot, cause necrosis only, chlorosis only or necrosis and chlorosis as symptoms of infection on appropriate wheat differentials. A toxin has been purified from a culture filtrate of a necrosis-only isolate. The toxin produces necrosis if it is infiltrated into the leaves of cultivars susceptible to the isolate but is without effect on cultivars resistant to the isolate. The toxin was purified by gel filtration and ion exchange chromatography and shown to be a protein. Sodium dodecyl sulphate polyacrylamide gel electrophoresis was used to show the toxin purity as well as estimate a protein molecular weight of 13900. The amino acid composition and molar extinction were determined. Proteolytic digestion or treatment of the purified toxin under denaturing conditions destroyed toxin activity. Infiltration of 10−13-10−14 moles of purified toxin into susceptible cultivars will produce necrosis.

References (25)

  • C.H.W. Hirs

    Determination of cystine as cysteic acid

  • K.J. Stevenson et al.

    Cerato-ulmin — a wilting toxin of Dutch elm disease fungus

    Phytochemistry

    (1979)
  • S. Blackburn

    Accuracy and sensitivity of ion exchange resin methods

  • CRC
  • J.M. Daly et al.

    Chemistry and biology of pathotoxins exhibiting non-selectivity

  • O.D. Dhingra et al.

    Basic Plant Pathology Methods

  • S.T. Gilchrist et al.

    Determination de resistencia contra Helminthosporium tritici-repentis bajo conditiones de campo e invernadero

    Agrociencia

    (1984)
  • R.N. Goodman et al.
  • W.A.F. Hagborg

    A device for injecting solutions and suspensions into thin leaves of plants

    Canadian Journal of Botany

    (1970)
  • R.M. Hosford

    Tan spot

  • J.M. Krupinsky

    Observations on the host range of isolates of Pyrenophora trichostoma

    Canadian Journal of Plant Pathology

    (1982)
  • J.M. Krupinsky

    Pathogenicity on wheat of Pyrenophora tritici-repentis isolated from Bromus inermis

    Phytopathology

    (1987)
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    Contribution number 824 from Plant Science Department, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, Canada.

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