High-performance liquid chromatographic determination, with ultraviolet detection, of S-adenosyl-l-methionine and S-adenosyl-l-homocysteine in rat tissues and simultaneously of normetanephrine and metanephrine for phenylethanolamine-N-methyltransferase or catechol-O-methyltransferase activities

Abstract

A high-performance liquid chromatographic method, with ultraviolet detection, for the determination of S-adenosyl-l-methionine and S-adenosyl-l-homocysteine, and simultaneously of normetanephrine and metanephrine, is presented. The separation was carried out by reversed-phase ion-pair isocratic chromatography. This procedure was applied to the study of the content of S-adenosyl-l-methionine and S-adenosyl-l-homocysteine in rat brain and adrenal glands and to the measurement of phenylethanolamine-N-methyltransferase or catechol-O-methyltransferase activities in rat adrenal gland.