Elsevier

Gene

Volume 147, Issue 2, 30 September 1994, Pages 169-177
Gene

Characterization of the mouse Rep-3 gene: sequence similarities to bacterial and yeast mismatch-repair proteins

https://doi.org/10.1016/0378-1119(94)90062-0Get rights and content

Abstract

The mouse Rep-3 gene is transcribed divergently from the same promoter region as the dihydrofolate reductase-encoding gene and has a deduced amino-acid sequence that shares identity with the bacterial protein, MutS, which is involved in DNA mismatch repair. We have cloned Rep-3, mapped it and sequenced all of the known exons and their intron junction sequences. We find that the open reading frame is considerably larger than initially reported and that the most abundant form of Rep-3 mRNA encodes a protein of 123 kDa. The gene spans at least 134 kb and consists of 26 exons, including several alternatively spliced exons. All of the exon/intron junctions match the expected consensus sequences with the exception of the splice junctions for intron 6, which has AT and AC dinucleotides instead of the usual GT and AG bordering the exon sequences. The junction sequences for this intron share consensus sequences with three intron sequences from other genes, thereby helping to establish an alternative consensus sequence.

References (39)

  • G.F. Crouse et al.

    Analysis of the mouse dhfr promoter region: existence of a divergently transcribed gene

    Mol. Cell. Biol.

    (1985)
  • J. Devereux et al.

    A comprehensive set of sequence analysis programs for the VAX

    Nucleic Acids Res.

    (1984)
  • B.W. Glickman et al.

    Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction

  • L.T. Haber et al.

    Nucleotide sequence of the Salmonella typhimurium mutS gene required for mismatch repair: homology of MutS and HexA of Streptococcus pneumoniae

    J. Bacteriol.

    (1988)
  • L.T. Haber et al.

    Altering the conserved nucleotide binding motif in the Salmonella typhimurium MutS mismatch repair protein affects both its ATPase and mismatch binding activities

    EMBO J.

    (1991)
  • I.J. Jackson

    A reappraisal of non-consensus mRNA splice sites

    Nucleic Acids Res.

    (1991)
  • R.G. Larson et al.

    Genomic structure of the human proliferating cell nucleolar protein P120

    Cancer Commun.

    (1990)
  • J.P. Linton et al.

    Dual bidirectional promoters at the mouse dhfr locus: cloning and characterization of two mRNA classes of the divergently transcribed Rep-1 gene

    Mol. Cell. Biol.

    (1989)
  • P.F.R. Little

    Choice and use of cosmid vectors

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    Present addresses: (K.L.) Advanced Tissue Sciences, 10933 N. Torrey Pines Road, La Jolla, CA 92037. USA. Tel. (1-619) 551-1912.

    ∗∗

    Present addresses: (J.P.L.) Linton Biotechnology Consulting, 479 Bristol Avenue, Cardiff, CA 92007, USA. Tel. (1-619) 942-3852.

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