Transformation of Penicillium chrysogenum to sulfonamide resistance
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Cited by (13)
Strain improvement for cephalosporin production by Acremonium chrysogenum using geneticin as a suitable transformation marker
2004, FEMS Microbiology LettersCitation Excerpt :There are few selection markers for transformation in A. chrysogenum. They include phleomycin [7,8], hygromycin B [8–10], and benomyl [11], whereas additional selection markers such as sulfonamide [12], oligomycin [13,14], acetamide [15], G418 [16], and auxotrophic complementation [17–23] have been described in the filamentous fungi Penicillium chrysogenum, Aspergillus nidulans and Neurospora crassa. Although the use of the aminoglycoside antibiotic G418 was described as a selection marker in Cephalosporium acremonium [24] using the APH(3′)I gene from Tn903, the transformation efficiency was very poor compared to the method described here involving the expression of the nptII geneticin resistance gene from Tn5 under a strong fungal promoter.
Mitotic instability of integrated plasmids in Penicillium chrysogenum transformants
1992, Journal of BiotechnologyAdvances, Problems, and Prospects of Genetic Transformation of Fungi
2018, Cytology and Genetics
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