Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis

doi:10.1016/0378-1119(83)90040-9

Gene

Volume 26, Issue 1, December 1983, Pages 101-106

Dedicated to Fred Sanger on the occasion of his retirement as a director of the MCR; Cambridge, UK.

https://doi.org/10.1016/0378-1119(83)90040-9 Get rights and content

Abstract

The restriction endonuclease cleavage sites for SphI and KpnI have been added to the lac cloning region of the phage vectors M 13mp10 and M 13mp11, using oligodeoxynucleotide-directed in vitro mutagenesis. Complementary deoxy 16-, 21- or 18-mers with the desired base changes were annealed to the M13mp DNA strand and extended with the Klenow fragment of DNA polymerase I. In adding these sites we have shown that this technique can be used as a general method for inserting sequences of DNA as well as introducing deletions and base pair changes.

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Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis

Abstract

Tetrahedron Lett.

Biochem. Biophys. Res. Commun.

Gene

Gene

Gene

Gene

Trends Biotechnol.

Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sites

Nature

Mutagenesis at a specific position in a DNA sequence

J. Biol. Chem.