Regular paper
A novel 550-kDa protein in skeletal muscle of chick embryo: purification and localization

https://doi.org/10.1016/0304-4165(95)00096-TGet rights and content

Abstract

We have found a novel protein with a molecular mass of 550 kDa on SDS-polyacrylamide gels, which is abundant in skeletal muscle tissues at an early stage of chick embryonic development. The 550-kDa protein decreased with the progress of development, and only a slight amount of the protein was present in adult chicken skeletal muscle. The 550-kDa protein was purified from the cytoplasm of 18 day embryos by a procedure including ultracentrifugation and gel filtration. The purified 550-kDa protein was essentially free of contaminants as judged by SDS-PAGE. By immunofluorescence and immunoelectron microscopy using the antibody raised against the 550-kDa protein, this protein was shown to be localized in the peripheries of adult muscle fibers and at the Z-disks of isolated myofibrils. These findings have led us to conclude that the 550-kDa protein is a novel myofibrillar protein in chicken skeletal muscle.

References (56)

  • B Vertel et al.

    Dev. Biol.

    (1976)
  • H Jockusch et al.

    Dev. Biol.

    (1980)
  • S.F Konieczny et al.

    Dev. Biol.

    (1982)
  • S.E Handel et al.

    Dev. Biol.

    (1989)
  • R Tatsumi et al.

    Anal. Biochem.

    (1995)
  • R.R Markwald

    J. Mol. Cell Cardiol.

    (1973)
  • D.A Fischman
  • B Geiger

    Cell.

    (1979)
  • M.G Price

    J. Biol. Chem.

    (1993)
  • E Lazarides et al.

    Cell

    (1978)
  • H.B Peng et al.

    Dev. Biol.

    (1981)
  • H Holtzer et al.

    Exp. Biol. Med.

    (1984)
  • B.L Grove et al.

    J. Cell Biol.

    (1985)
  • D.A Fischman
  • C.S Hill et al.

    J. Cell Biol.

    (1986)
  • H.F Epstein et al.

    Science

    (1991)
  • H Holtzer et al.

    J. Byophys. Biochem. Cytol.

    (1957)
  • K Okazaki et al.

    J. Cytol. Histochem.

    (1965)
  • J.C Chi et al.
  • J.C Chi et al.

    J. Cell Biol.

    (1975)
  • C.S Hill et al.

    J. Cell Biol.

    (1986)
  • N.J Colley et al.

    J. Cell Sci.

    (1990)
  • A.A Dlugosz et al.

    J. Cell Biol.

    (1984)
  • P.B Antin et al.

    J. Cell Biol.

    (1986)
  • K.T Tokuyasu et al.

    J. Cell Biol.

    (1987)
  • K.T Tokuyasu et al.

    J. Cell Biol.

    (1987)
  • D.O Fürst et al.

    J. Cell Biol.

    (1989)
  • Z Lin et al.

    J. Cell Biol.

    (1989)

    • Cited by (4)

    • Gel electrophoresis for studying biological function

      1998, Analytica Chimica Acta

    • Biochemical factors regulating the toughening and tenderization processes of meat

      1996, Meat Science

    • Monitoring post mortem changes in porcine muscle through 2-D DIGE proteome analysis of Longissimus muscle exudate

      2013, Proteome Science

    • The different pattern of protein distribution in two skeletal muscles of Japanese quail during ontogenesis

      1998, Chemical Papers
    View full text
    Copyright © 1995 Published by Elsevier B.V.