The morphology of ultrathin cryosections
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Towards high-resolution three-dimensional imaging of native mammalian tissue: Electron tomography of frozen-hydrated rat liver sections
2006, Journal of Structural BiologyCutting artefacts and cutting process in vitreous sections for cryo-electron microscopy
2005, Journal of Structural BiologyCitation Excerpt :It is also a well-known artefact in classical sections, though it could have there a different origin. There are numerous articles dealing with the cryosectioning process or directly relevant to it (Chang et al., 1983; Dubochet and McDowall, 1984; Dubochet et al., 1988; Hsieh et al., 2002; Jésior, 1986; Lickfeld, 1985; McDowall et al., 1983; Richter et al., 1991; Richter, 1994a,b,c; Sartori et al., 1993; Sartori Blanc et al., 1998; Sitte, 1996; Studer et al., 2001; Zhang et al., 2004; Zierold, 1984, 1987) but many aspects remains uncertain or not understood. It is the aim of the present study to analyse in more detail the cutting artefacts and the cutting process and determine the optimal conditions for producing high quality vitreous sections.
Cryoelectron microscopy of refrozen cryosections
2003, Journal of Structural BiologyTransverse sectioning of plastic-embedded immunolabeled cryosections: Morphology and permeability to protein A-colloidal gold complexes
1986, Journal of Ultrastructure Research and Molecular Structure ResearchHow to avoid compression II. The influence of sectioning conditions
1986, Journal of Ultrastructure Research and Molecular Structure Research