Elsevier

Neuroscience Letters

Volume 212, Issue 3, 19 July 1996, Pages 159-162
Neuroscience Letters

A μ-receptor opioid agonist induces AP-1 and NF-κB transcription factor activity in primary cultures of rat cortical neurons

https://doi.org/10.1016/0304-3940(96)12799-3Get rights and content

Abstract

Activator protein 1 (AP-1) and nuclear factor κB (NF-κB) represent mammalian transcription factors which bind to distinct enhancer motifs. The specific μ-receptor opioid agonist, Tyr, d-Ala2, Gly, N-Me-Phe4, Gly-ol5 (DAMGO), was found to increase AP-1 and NF-κB activity in primary cultures of neurons from rat cerebral cortex. Acute (2 h, 4 h) and long-term (72 h) treatment with DAMGO time-dependently increased the DNA-binding activity of both AP-1 and NF-κB and the stimulation could be abolished or inhibited by concurrent incubation with naloxone. However, acute naloxone-precipitated withdrawal did not significantly change AP-1 or NF-κB activity. These results indicate a μ-opioid receptor-related co-induction of AP-1 and NF-κB transcription factors in cultured cortical neurons.

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