Bioaccumulation of heavy metals in Escherichia coli expressing an inducible synthetic human metallothionein gene
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Role of biological macromolecules of microbes in metal bioremediation
2020, Microbial and Natural Macromolecules: Synthesis and ApplicationsImplication of plants and microbial metalloproteins in the bioremediation of polluted waters: A review
2014, Physics and Chemistry of the EarthCitation Excerpt :Problems may arise following intracellular expression of MT and these may be linked to the stability and short half-life of the expressed heterologous proteins (Mejare and Bulow, 2001). However, solution to these problems consists of the expression of a fusion protein (Romeyer et al., 1988). The hexapeptide (HSQKVF) was expressed as a fusion to the exposed area of the OmpA in E. coli improved cells resistance to toxic concentrations of Cd (Mejare and Bulow, 2001).
State-of-the-art of metallothioneins at the beginning of the 21st century
2012, Coordination Chemistry ReviewsCitation Excerpt :First attempts to recombinantly express MT genes were carried out for mammalian isoforms in E. coli [29,30], but had limited success, probably because of stability problems of these peculiar proteins in a prokaryotic environment. Alternatively, fusion protein systems were attempted, which immediately improved the recovery yield [31–33]. Yeast (S. cerevisiae) was also assayed as alternative eukaryotic heterologous host, with positive results for Drosophila and mammalian MTs [34,35].
Promotion of metal accumulation in nodule of Astragalus sinicus by the expression of the iron-regulated transporter gene in Mesorhizobium huakuii subsp. rengei B3
2008, Journal of Bioscience and Bioengineering