The use of a two-liquid-phase electron removal system for culture of proton-reducing bacteria

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Abstract

An artificial electron removal system was tested for the culture of proton-reducing bacteria. A chemically modified hydrophilic viologen derivative propylviologen sulphonate (PVS) functioned as redox mediator, and 2-anilino-1, 4-naphthoquinone dissolved in dibutylphthalate was the terminal electron acceptor. To test the quality of the electron removal system, the organism Acidaminobacter hydrogenoformans was employed. The products formed during glutamate fermentation by this organism depend on the hydrogen partial pressure. Results show that electron transfer to 2-anilino-1,4-naphthoquinonoxe is possible. However, a shift to formation of propionate, a product which is produced at a low hydrogen partial pressure, was not found.

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