Fluorescent microparticles as a rapid tool in bacterial adherence studies

doi:10.1016/0167-7012(91)90013-G

Journal of Microbiological Methods

Volume 13, Issue 2, June 1991, Pages 135-143

https://doi.org/10.1016/0167-7012(91)90013-G Get rights and content

Abstract

Fluorescent microparticles (FMPs) coated with the O75X adhesin of uropathogenic Escherichia coli were evaluated for use in adhesion studies. The purified o75X adhesin coated efficiently onto the microparticles, and the O75X-FMPs retained the agglutination properties of the intact O75X adhesin molecules. Binding tests on frozen sections of human kidney and with immobilized target proteins showed that the tissue- and molecular-binding specificities of the O75X-FMPs were closely similar to those of the intact O75X adhesin. The results show that adhesin-coated FMPs are rapid and stable reagents for functional study of bacterial adhesion proteins.

References (31)

V. Väisänen-Rhen et al.
Adhesion of Escherichia coli to human kidney cryostat sections
FEMS Microbiol. Lett.
(1985)
B. Nowicki et al.
Location of adhesion sites for P-fimbriated and O75X-positive Escherichia coli in the human kidney
Microb. Pathogen.
(1986)
G.C. Hansson et al.
Carbohydrate-specific adhesion of bacteria to thin-layer chromatograms: a rationalized approach to the study of host cell glycolipid receptors
Anal. Biochem.
(1985)
R. Virkola
Binding characteristics for Escherichia coli type-1 fimbriae in the human kidney
FEMS Microbiol. Lett.
(1987)
J. Mirro et al.
Simultaneous analysis of cell surface antigens and cell morphology using monoclonal antibodies conjugated to fluorescent microspheres
J. Immunol. Methods
(1981)
T.J. Higgins et al.
A sensitive and quantitative fluorescence assay for cell surface antigens
J. Immunol. Methods
(1981)
T.K. Korhonen
Yeast cell agglutination by purified enterobacterial pili
FEMS Microbiol. Lett.
(1979)
B. Westerlund et al.
A novel lectin-independent interaction of P fimbriae of Escherichia coli with immobilized fibronectin
FEBS Lett.
(1989)
B. Westerlund et al.
The O75X adhesin of uropathogenic Escherichia coli receptor-active domains in the canine urinary tract and in vitro interaction with laminin
Microb. Pathogen.
(1987)
R. Glanville
Type IV collagen

B. Nowicki et al.

Kinetics of phase variation between S and type-1 fimbriae of Escherichia coli

FEMS Microbiol. Lett.

(1985)

C. Svanborg-Edén et al.

Adhesion of Escherichia coli to human uroepithelial cells in vitro

Infect. Immun.

(1977)

T.K. Korhonen et al.

Binding specificity of piliated strains of Escherichia coli and Salmonella typhimurium to epithelial cells, Saccharomyces cerevisiae cells, and erythrocytes

Infect. Immun.

(1981)

E. Garcia et al.

Adhesion of canine and human uropathogenic Escherichia coli and Proteus mirabilis strains to canine and human epithelial cells

Curr. Microbiol.

(1988)

D.A. Powell et al.

Attachment of Mycoplasma pneumoniae to respiratory epithelium

Infect. Immun.

(1976)

Cited by (7)

Binding of bacterial adhesins to rat glomerular mesangium in vivo
1993, Kidney International
Binding of bacterial adhesins to rat glomerular mesangium in vivo. Two well characterized bacterial adhesins, the O75X fimbriae of Escherichia coli and the type-3 fimbriae of Klebsiellae, with in vitro affinities to type IV and V collagens, respectively, were used to test whether bacterial components with affinity for glomerular matrix could bind to glomeruli in vivo. The purified fimbrial proteins were injected into rats, and kidney samples were studied by immunofluorescence at two hours to nine months postinjection. The O75X, but not the type-3 fimbriae, formed mesangial deposits that persisted for months. Preincubation of the O75X fimbriae with type IV collagen significantly reduced the glomerular binding. The fimbrial deposits were extracellular, as anti-O75X IgG injected into rats bound to glomeruli. Proteinuria or histological damage could not be detected even after passive or active immunizations of the rats. The results demonstrate that bacterial adhesins may bind in vivo to and persist in glomeruli by their specific affinities. The results also indicate that additional factors provided by the bacteria or the host are needed for glomerular damage to take place.
Biofilms: Methods for enzymatic release of microorganisms
2017, Biofilms: Methods for Enzymatic Release of Microorganisms
Identification of a high-molecular-mass Lactobacillus epithelium adhesin (LEA) of Lactobacillus crispatus ST1 that binds to stratified squamous epithelium
2012, Microbiology (United Kingdom)
pH-dependent association of enolase and glyceraldehyde-3-phosphate dehydrogenase of Lactobacillus crispatus with the cell wall and lipoteichoic acids
2007, Journal of Bacteriology
Adhesive properties of the purified plasminogen activator Pla of Yersinia pestis
2006, FEMS Microbiology Letters
Demonstration of adherence properties of Porphyromonas gingivalis outer membrane vesicles using a new microassay
1995, Oral Microbiology and Immunology

View all citing articles on Scopus

View full text

Article preview

Journal of Microbiological Methods

Abstract

References (31)

Adhesion of Escherichia coli to human kidney cryostat sections

FEMS Microbiol. Lett.

Location of adhesion sites for P-fimbriated and O75X-positive Escherichia coli in the human kidney

Microb. Pathogen.

Carbohydrate-specific adhesion of bacteria to thin-layer chromatograms: a rationalized approach to the study of host cell glycolipid receptors

Anal. Biochem.

Binding characteristics for Escherichia coli type-1 fimbriae in the human kidney

FEMS Microbiol. Lett.

Simultaneous analysis of cell surface antigens and cell morphology using monoclonal antibodies conjugated to fluorescent microspheres

J. Immunol. Methods

A sensitive and quantitative fluorescence assay for cell surface antigens

J. Immunol. Methods

Yeast cell agglutination by purified enterobacterial pili

FEMS Microbiol. Lett.

A novel lectin-independent interaction of P fimbriae of Escherichia coli with immobilized fibronectin

FEBS Lett.

The O75X adhesin of uropathogenic Escherichia coli receptor-active domains in the canine urinary tract and in vitro interaction with laminin

Microb. Pathogen.

Type IV collagen

Kinetics of phase variation between S and type-1 fimbriae of Escherichia coli

FEMS Microbiol. Lett.

Adhesion of Escherichia coli to human uroepithelial cells in vitro

Infect. Immun.

Binding specificity of piliated strains of Escherichia coli and Salmonella typhimurium to epithelial cells, Saccharomyces cerevisiae cells, and erythrocytes

Infect. Immun.

Adhesion of canine and human uropathogenic Escherichia coli and Proteus mirabilis strains to canine and human epithelial cells

Curr. Microbiol.

Attachment of Mycoplasma pneumoniae to respiratory epithelium

Infect. Immun.

Cited by (7)

Binding of bacterial adhesins to rat glomerular mesangium in vivo

Biofilms: Methods for enzymatic release of microorganisms

Identification of a high-molecular-mass Lactobacillus epithelium adhesin (LEA) of Lactobacillus crispatus ST1 that binds to stratified squamous epithelium

pH-dependent association of enolase and glyceraldehyde-3-phosphate dehydrogenase of Lactobacillus crispatus with the cell wall and lipoteichoic acids

Adhesive properties of the purified plasminogen activator Pla of Yersinia pestis

Demonstration of adherence properties of Porphyromonas gingivalis outer membrane vesicles using a new microassay