MHC class II compatibility in aborted fetuses and term infants of couples with recurrent spontaneous abortion

doi:10.1016/0165-0378(93)90063-N

Journal of Reproductive Immunology

Volume 25, Issue 3, December 1993, Pages 195-207

https://doi.org/10.1016/0165-0378(93)90063-N Get rights and content

Abstract

Maternal-fetal histocompatibility for alleles at HLA class II loci, HLA-DQA1 and HLA-DQB1, was examined in 40 abortuses and 31 liveborn children of 68 couples with a history of idiopathic recurrent spontaneous abortion (RSAB) who underwent leukocyte immunization prior to the index pregnancy. Significantly more couples with RSAB shared two HLA-DQA1 alleles as compared with fertile control couples (0.18 vs. 0.03, respectively; P = 0.031). There were no differences in HLA sharing between couples with RSAB who experienced a repeat abortion in the index pregnancy as compared with couples with RSAB who were delivered of a liveborn child. Non-significant deficits of abortuses who were compatible for alleles at the HLA-DQA1 (6 observed vs. 8.5 expected; P = 0.225) and the HLA-DQB1 (7 observed vs. 9.2 expected; P = 0.254) loci were observed. A significant deficit of HLA-DQA1 compatible liveborn children was observed (1 observed vs. 5.5 expected; P = 0.0069). The overall deficit of HLA-DQA1 compatible fetuses (7 observed vs. 14.0 expected; P = 0.0018) after approximately 8 weeks gestation suggests that HLA-DQA1 compatible fetuses may be aborted early in pregnancy, prior to the time when fetal tissue can be recovered for genetic studies.

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Cited by (52)

HLA associations and HLA sharing in recurrent miscarriage: A systematic review and meta-analysis
2015, Human Immunology
Citation Excerpt :
All studies included for meta-analyses on 14-bp genotype included screening for APS and therefore this could not explain the heterogeneity observed. In total 21 eligible studies reported on classical HLA I sharing (HLA-A, HLA-B, HLA-C sharing) and classical HLA II sharing (HLA-DR, HLA-DQ, HLA-DP sharing) in couples with RM [45,50,53–56,58–64,70–77]. For HLA-A, B, C sharing most studies used serological typing method, except one study [61] which used molecular method to define HLA-C alleles.
The aim of this meta-analysis was to evaluate whether specific maternal HLA alleles and HLA sharing of couples are associated with the occurrence of recurrent miscarriage (RM).
A systematic literature search was performed for studies that evaluated the association between HLA alleles, HLA sharing and RM. RM was defined as three or more consecutive unexplained miscarriages and a control group was included of women with at least one live birth and no miscarriages in their history. Meta-analyses were performed and the pooled odds ratio (OR) was calculated.
We included 41 studies. Selection bias was present in 40 studies and information bias in all studies. Meta-analyses showed an increased risk of RM in mothers carrying a HLA-DRB1*4 (OR 1.41, 95% CI 1.05–1.90), HLA-DRB1*15 (OR 1.57, 95% CI 1.15–2.14), or a HLA-E*01:01 allele (OR 1.47, 95% CI 0.20–1.81), and a decreased risk with HLA-DRB1*13 (OR 0.63, 95% CI 0.45–0.89) or HLA-DRB1*14 (OR 0.54, 95% CI 0.31–0.94). Pooling results for HLA sharing showed that HLA-B sharing (OR 1.39, 95% CI 1.11–1.75) and HLA-DR sharing (OR 1.57, 95% CI 1.10–1.25) were both associated with the occurrence of RM.
Although the present systematic review and meta-analysis demonstrates that specific HLA alleles and HLA sharing are associated with RM, a high degree of bias was present and therefore observed results should be interpreted carefully.
Molecular genetic studies on the compatibility of HLA class II alleles in patients with unexplained recurrent miscarriage in the Japanese population
2006, Clinical Immunology
It is hypothesized that patients having unexplained recurrent miscarriage lack an appropriate immune reaction against their partner's antigens, which means possible compatibility of HLA antigens between the patient couples. The conclusion, however, has not yet been achieved, so the purpose was to determine whether significant compatibility of HLA class II exists between the couples. The HLA-DRB1 and -DQB1 genotypes were determined using PCR-RFLP method in 91 patient couples and in 72 normal couples. The number of patient couples with zero-allele mismatch was not significantly different compared with that of control couples regarding HLA-DRB1 genotype and phenotype, as well as regarding HLA-DQB1 genotype and phenotype. While the number of patient couples with zero- and one-allele mismatch was significantly higher compared with that in control as to HLA-DR and -DQ phenotype (P = 0.029 by Chi-square test). In conclusion, it is suggested that the compatibility of HLA class II antigens between couples might be involved in the genesis of unexplained recurrent miscarriage.
The badgers of Wytham woods: A model for behaviour, ecology, and evolution
2022, The Badgers of Wytham Woods: A Model for Behaviour, Ecology, and Evolution
HLA System and its Participation in Recurrent Pregnancy Loss
2022, Pakistan Journal of Zoology
Clinical molecular genetics evaluation in women with reproductive failures
2021, American Journal of Reproductive Immunology
Inflammatory markers and proteomic analysis
2018, Recurrent Pregnancy Loss

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MHC class II compatibility in aborted fetuses and term infants of couples with recurrent spontaneous abortion

Abstract

Lancet

Pregnancy outcome in couples with recurrent abortions following immunological evaluation and therapy

The Immunobiology of Mammalian Reproduction

Pregnancy outcome in human couples with recurrent spontaneous abortions: HLA antigen profiles, female serum MLR block factors, and paternal leukocyte immunization

Exp. Clin. Immunogenet.

Polymorphic DNA region adjacent to the 5′ end of the human insulin gene

Nomenclature for factors of the HLA system

Human Immunol.

Prenatal diagnosis using DNA polymorphisms

Rapid typing of HLA-DQB1 polymorphism using nonradioactive oligonucleotide probes and amplified DNA

Immunogenetics

Paternal mononuclear cell immunization therapy for repeated miscarriage: predictive variables for pregnancy success

Am. J. Reprod. Immunol.