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Countercurrent chromatography

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Abstract

Countercurrent chromatography is a newly developed versatile partition chromatography which totally eliminates the use of solid supports. The method utilizes the intriguing hydrodynamic behavior of two immiscuble solvents in a rotating coiled tube. Various types of seal-free flow-through centrifuge schemes are introduced to facilitte a continuous elution process. The method yields high partition efficiencies comparable to liquid chromatography but without the complications arising the use of solid supports. Countercurrent chromatography covers a wid spectrum of applicable samples ranging from small ions and molecules to macromolecules and even cell particles in both analytical and preparative scales.

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