Epitope expression of gonococcal lipooligosaccharide (LOS). Importance of the lipoidal moiety for expression of an epitope that exists in the oligosaccharide moiety of LOS
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Cited by (26)
Human lipooligosaccharide IGG that prevents endemic meningococcal disease recognizes an internal lacto-N-neotetraose structure
2011, Journal of Biological ChemistryCitation Excerpt :MS of intact LOS of the group C strain, 89I, did find multiple lipid phosphoforms (42, 43), but this might have been an exception. What effects, if any, the different lipids might have on glycose antigen formation (48, 49) were not explored. Also surprising was that the nLc4 terminal Gal appeared to protect L3,7 but not L2,4 strains from bactericidal activity.
Selection of peptides that bind to the core oligosaccharide of R-form LPS from a phage-displayed heptapeptide library
2001, FEMS Microbiology LettersStructural relationships and sialylation among meningococcal L1, L8, and L3,7 lipooligosaccharide serotypes
2000, Journal of Biological ChemistryCitation Excerpt :We dephosphorylated LOS and OS by hydrolysis with HF. LOS and OS were reacted with a 48% (aqueous) HF solution (10 μg of LOS per μl) in the dark at 4 °C for 16–20 h (OS and O-deacylated LOS) or 36–40 h (intact LOS) (9). After evaporation of HF, the residues were resuspended in H2O and lyophilized.
Separation of lipooligosaccharides by linear gradient gel electrophoresis
2000, Analytical Biochemistry
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