Expression cloning of 2-5A-dependent RNAase: A uniquely regulated mediator of interferon action

doi:10.1016/0092-8674(93)90403-D

Cell

Volume 72, Issue 5, 12 March 1993, Pages 753-765

https://doi.org/10.1016/0092-8674(93)90403-D Get rights and content

Abstract

2-5A-dependent RNAase, an interferon-induced enzyme that is activated by 5′-phosphorylated, 2′,5′-linked oligoadenylates (2-5A), is implicated in both the molecular mechanisms of interferon action and the fundamental control of RNA stability in mammalian cells. Here we report the expression cloning and analysis of murine and human 2-5A-dependent RNAases. The 2-5A binding properties and RNAase activities of recombinant and naturally occurring forms of 2-5A-dependent RNAase were identical. Interferon induction of 2-5A-dependent RNAase expression was demonstrated by measuring the mRNA levels in cells treated with interferon and cycloheximide. Analysis of aligned murine and human 2-5A-dependent RNAase sequences revealed several intriguing features, including similarity to RNAase E, which is implicated in the control of mRNA stability in E. coli. Interestingly, a duplicated phosphate-binding loop motif was determined by deletion analysis and site-directed mutagenesis to function in the binding of 2-5A.

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Cell

ArticleExpression cloning of 2-5A-dependent RNAase: A uniquely regulated mediator of interferon action

Abstract

J. Biol. Chem.,

Cell

Biochem. Biophys. Res. Commun.,

Anal. Biochem.,

J. Biol. Chem.,

Cell

J. Biol. Chem.,

Cell

Virology

J. Biol. Chem.,

Virology

J. Biol. Chem.,

Cell

J. Biol. Chem.,

J. Biol. Chem.,

Anal. Biochem.,

J. Mol. Biol.,

Trends Biochem. Sci.,

J. Biol. Chem.,

Cell

Anal. Biochem.,

J. Biol. Chem.,

Cell

J. Biol. Chem.,

J. Biol. Chem.,

The Ams (altered mRNA stability) protein and ribonuclease E are encoded by the same structural gene of Escherichia coli

(p)pp(A2′p)nA is rare in normal mouse tissue while (A2′p)nA but not (p)pp(A2′p)nA appears to be present in E. coli

Prog. Clin. Biol. Res.,

Constitutive expression of (2′–5′) oligo A synthetase confers resistance to picornavirus infection

Nature

Specificity of Escherichia coli endoribonuclease RNAase E: in vivo and in vitro analysis of mutants in a bacteriophage T4 mRNA processing site

Genes Dev.,

(2′–5′)oligoadenylate in rat liver: modulation after partial hepatectomy

(2′–5′)An-dependent endoribonuclease: enzyme levels are regulated by IFNβ, IFNγ, and cell culture conditions

J. Cell. Biochem.,

Article
Expression cloning of 2-5A-dependent RNAase: A uniquely regulated mediator of interferon action

(p)pp(A2′p)_nA is rare in normal mouse tissue while (A2′p)_nA but not (p)pp(A2′p)_nA appears to be present in E. coli

(2′–5′)A_n-dependent endoribonuclease: enzyme levels are regulated by IFNβ, IFNγ, and cell culture conditions