Cell
Volume 62, Issue 6, 21 September 1990, Pages 1053-1062
Journal home page for Cell

Article
A kinesin-like protein required for distributive chromosome segregation in Drosophila

https://doi.org/10.1016/0092-8674(90)90383-PGet rights and content

Abstract

The nod gene is required for the distributive segregation of nonexchange chromosomes during meiosis in D. melanogaster. Loss-of-function nod mutations cause nondisjunction and loss of nonrecombinant chromosomes both at melosis I and during subsequent mitotic divisions. We have cloned the nod locus, examined its expression patterns, and determined its coding sequence. In adults the nod transcript is only present in females, consistent with the observation that males do not use the distributive segregation system. However, the nod locus is also transcribed in the embryonic, larval, and pupal stages of development, and possibly in all dividing cells. Finally, the N-terminal domain of the predicted nod protein has amino acid similarity to the mechanochemical domain of kinesin heavy chain; however, the C-terminal domain is unlike that of kinesin heavy chain or of any previously reported protein. Thus, the nod protein is a member of the kinesin superfamily and may be a microtubule motor.

References (45)

  • J.R. Bell et al.

    A new copia-like transposable element found in a Drosophila rDNA gene unit

    Nucl. Acids Res.

    (1985)
  • A.T.C. Carpenter

    A mutant defective in distributive disjunction in Drosophila melanogaster

    Genetics

    (1973)
  • D.R. Cavener

    Comparison of the consensus sequence flanking translational start sites in Drosophila and vertebrates

    Nucl. Acids Res.

    (1987)
  • D.G. Davis

    Chromosome behavior under the influence of claret-nondisjunctional in Drosophila melanogaster

    Genetics

    (1969)
  • J. Devereux et al.

    A comprehensive set of sequence analysis programs for the VAX

    Nucl. Acids Res.

    (1984)
  • S.A. Endow et al.

    A kinesin-related protein mediates meiotic and early mitotic chromosome segregation in Drosophila

    Nature

    (1990)
  • R.F. Grell

    Distributive pairing

  • R.S. Hawley

    Exchange and chromosomal segregation in eucaryotes

  • R.S. Hawley

    Genetic and molecular analysis of a simple disjunctional system in Drosophila melanogaster

  • A. Himmler et al.

    Tau consists of a set of proteins with repeated C-terminal microtubule-binding domains and variable N-terminal domains

    Mol. Cell. Biol.

    (1989)
  • P.F. Johnson et al.

    Eukaryotic transcriptional regulatory proteins

    Annu. Rev. Biochem.

    (1989)
  • R.C. King

    Ovarian Development in Drosophila melanogaster

    (1970)
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