Cell
Volume 37, Issue 1, May 1984, Pages 123-129
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Article
Nucleotide sequence analysis identifies the human c-sis proto-oncogene as a structural gene for platelet-derived growth factor

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Abstract

The simian sarcoma virus transforming gene, v-sis, encodes a protein, p28sis, that is closely related to human platelet-derived growth factor (PDGF). The human locus related to v-sis was cloned and shown to contain at least five exons corresponding to the v-sis coding region. Nucleotide sequence analysis of these exons revealed that the predicted amino acid sequence of human c-sis differed by 6% from that of the woolly monkey-derived v-sis. These findings imply that the sis proto-oncogene has been well conserved during primate evolution. By comparison of the known amino acid sequences of PDGF peptides with the predicted human c-sis protein, it was possible to demonstrate that this human proto-oncogene is the structural gene encoding one of the two major polypeptides of this potent mitogen for connective tissue cells.

References (53)

  • P.A. Sharp

    Speculations on RNA splicing

    Cell

    (1981)
  • G.L.C. Shen-Ong et al.

    Novel myc oncogene RNA from abortive immunoglobulin-gene recombination in mouse plasmacytomas

    Cell

    (1982)
  • E.M. Southern

    Detection of specific sequences among DNA fragments separated by gel electrophoresis

    J. Mol. Biol.

    (1975)
  • K. Alitalo et al.

    Homogeneously staining chromosomal regions contain amplified copies of an abundantly expressed cellular oncogene (c-myc) in malignant neuro-endocrine cells from a human colon carcinoma

  • P.R. Andersen et al.

    Generation of Balb-MuSV and Ha-MuSV by type C virus transduction of homologous transforming genes from different species

    Cell

    (1981)
  • H.N. Antoniades et al.

    Human platelet-derived growth factor (PDGF): amino terminal amino acid sequence

    Science

    (1983)
  • W.D. Benton et al.

    Screening λgt recombinant clones by hybridization to single plaques in situ

    Science

    (1977)
  • P. Besmer et al.

    The Parodi-Irgens feline sarcoma virus and simian sarcoma virus have homologous oncogenes, but in different contexts of the viral genomes

    J. Virol.

    (1983)
  • J.M. Bishop

    Cellular oncogenes and retroviruses

    Ann. Rev. Biochem.

    (1983)
  • E. Canaani et al.

    Activation of the c-mos oncogene in a mouse plasmacytoma by insertion of an endogenous intracisternal A-particle genome

  • S. Collins et al.

    Amplification of endogenous myc-related DNA sequences in a human myeloid leukemia cell line

    Nature

    (1982)
  • R. Dalla-Favera et al.

    A human onc gene homologous to the transforming gene (v-sis) of simian sarcoma virus

    Nature

    (1981)
  • R. Dalla-Favera et al.

    Human c-myc onc gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma

  • R. Dalla-Favera et al.

    Onc gene amplification in promyelocytic leukemia cell line HL-60 and primary leukemia cells of the same patient

    Nature

    (1982)
  • D. De Feo-Jones et al.

    ras-related gene sequences identified and isolated from Saccharomyces cerevisiae

    Nature

    (1983)
  • S.G. Devare et al.

    Nucleotide sequence of the simian sarcoma virus genome: demonstration that its acquired cellular sequences encode the transforming gene product p28sis

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