Recombination within the yeast plasmid 2μ circle is site-specific

doi:10.1016/0092-8674(82)90107-6

Cell

Volume 29, Issue 1, May 1982, Pages 227-234

https://doi.org/10.1016/0092-8674(82)90107-6 Get rights and content

Abstract

The multicopy yeast plasmid, 2μ circle, encodes a specialized recombination system. It contains two regions, each 599 bp in length, that are precise inverted repeats of each other and between which recombination occurs readily. In addition, this recombination requires the product of a 2μ circle gene, designated FLP. By examining the products of FLP-mediated recombination of plasmids containing single insertions within one of the repeated regions, we show that this recombination occurs only at a specific site within the repeat. This result was confirmed from analysis of the ability of plasmids containing various deletions within one of the repeated regions to serve as substrates for FLP-mediated recombination. These experiments limit the recombination site to a sequence of less than 65 bp. In addition, by mutational analysis of the recombination potential of a hybrid plasmid containing the entire 2μ circle genome, we have shown that FLP is only the 2μ circle gene necessary for this site-specific recombination. Finally, we describe a sensitive assay for recombination between the repeated sequences of 2μ circle; using it, we demonstrate that even in the absence of FLP gene product, recombination between the repeats occurs at a low but detectable level during meiosis.

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Abstract

References (27)

Replication and recombination functions associated with the yeast plasmid, 2μ circle

Cell

Identification and mapping of the transcriptional and translational products of the yeast plasmid, 2μ circle

Cell

Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene

Gene

High frequency of yeast transformation by plasmids carrying part or entire 2-μm yeast plasmid

Gene

Transposon-mediated site-specific recombination: a defined in vitro system

Cell

The inverted repeats of Tn5 are functionally different

Cell

Isolation of a circular derivative of yeast chromosome III: implications for the mechanism of mating type interconversion

Cell

Transformation of yeast by a replicating hybrid plasmid

Nature

Occurrence of crossed strand-exchange forms in yeast DNA during meiosis

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

Nucl. Acids Res.

The invertible segment of bacteriophage Mu DNA determines the absorption properties of Mu particles

Nature

Isolation of a yeast centromere and construction of functional small circular chromosomes

Nature

Genetic properties of chromosomally integrated 2μ plasmid DNA in yeast

Cell

Cited by (161)

Genetic conflicts in budding yeast: The 2μ plasmid as a model selfish element

Intersectional mapping of multi-transmitter neurons and other cell types in the brain

Advances in peptide-mediated cytosolic delivery of proteins

A toolkit for DNA assembly, genome engineering and multicolor imaging for C. elegans

Engineering Pichia pastoris for improved NADH regeneration: A novel chassis strain for whole-cell catalysis

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Cell

ArticleRecombination within the yeast plasmid 2μ circle is site-specific

Abstract

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Gene

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Cell

Transformation of yeast by a replicating hybrid plasmid

Nature

Occurrence of crossed strand-exchange forms in yeast DNA during meiosis

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

Nucl. Acids Res.

The invertible segment of bacteriophage Mu DNA determines the absorption properties of Mu particles

Nature

Isolation of a yeast centromere and construction of functional small circular chromosomes

Nature

Genetic properties of chromosomally integrated 2μ plasmid DNA in yeast

Cell

Article
Recombination within the yeast plasmid 2μ circle is site-specific