Pharmacological modulation of prostacyclin and thromboxane production of rat and cat venous tissue slices

https://doi.org/10.1016/0090-6980(92)90007-GGet rights and content

Abstract

To reveal a potential modulating effect of vasoactive pharmacological agents on the prostanoid production of the venous wall, prostacyclin and thromboxane release from venous tissue slices was studied. Aortic and caval vein samples from 20 rats as well as from 21 cats were studied. Prostacyclin and thromboxane productions were determined by radioimmunoassay as 6-keto-PGF1 alfa and TxB2 released into the incubation medium. Venous tissue produced significantly less prostacyclin per unit weight than arterial tissue in rats (30.7±4.6 vs. 52.1±8.2 pg/mg/min), while in cats an opposite situation was found (16.6±3.2 vs. 7.06±1.9 pg/mg/min). Thromboxane production of venous tissue was consequently higher than corresponding values for aortic tissue (3.72±0.46 vs. 1.54±0.14 in rats and 3.4±0.6 vs. 1.33±0.19 in cats, all values in pg/mg/min). Norepinephrine and dopamine significantly increased both the prostacyclin and the thromboxane release from venous tissue, while isoproterenol had no effect. Vasopressin significantly increased thromboxane release and decreased the ratio of prostacyclin vs. thromboxane production (from 10.4±1.6 to 7.5±1.6, in acetylsalicylic acid pretreated cats). Angiotensin and thrombin had no significant effects. Bradykinin (0.5 μg/ml) significantly augmented prostacyclin release from venous tissue (14.4±2.6 from 10.9±2.4 pg/mg/min) and decreased thromboxane release (0.65±0.18 from 1.35±0.22 pg/mg/min). Methionin-enkephalin (5 μg/ml) significantly reduced the thromboxane release from venous tissue slices. The presented material demonstrates that several vasoactive agents modulate the vasoactive prostanoid release of the venous wall. In some cases, the prostacyclin and the thromboxane productions are influenced separately, which in turn will have its impact on smooth muscle activity and thrombocyte aggregation.

References (55)

  • O. Boutherin-Falson et al.

    Decreased prostacyclin production by human umbilical vein endothelial cells cultured with endothelial cell growth factor and heparin

    Thromb. Res.

    (1989)
  • T.F. Kresowik et al.

    Effect of preexcisional heparin anticoagulation on excised canine vein and artery prostanoid production

    J. Surg. Res.

    (1989)
  • G.R. Upchurch et al.

    Differences in secretion of prostacyclin by venous and arterial endothelial cells grown in vitro in a static versus a mechanically active environment

    J. Vasc. Surg.

    (1989)
  • E.D. Endean et al.

    Effect of thromboxane synthetase inhibition on canine autogenous vein grafts

    J. Surg. Res.

    (1986)
  • P. Hadhazy et al.

    Endogenous prostanoids and arterial contractility

    Prostagland. Leukotrien. Essent. Fatty Acids

    (1988)
  • O. Vesterqvist et al.

    Thromboxane and prostacyclin formation in patients with deep vein thrombosis

    Thromb. Res.

    (1987)
  • J.A. Andrade et al.

    Local plasminogen activator and prostacyclin-like activity after partial venous occlusion - an experimental study in the dog

    Thromb. Res.

    (1988)
  • S.L. Hong

    Effect of bradykinin and thrombin on prostacyclin synthesis in endothelial cells from calf and pig aorta and human umbilical cord vein

    Thromb. Res.

    (1980)
  • B.B. Weksler et al.

    Human leukocyte cathepsin G and elastase specifically suppress thrombin induced prostacyclin production in endothelial cells

    Blood

    (1989)
  • R.W. Walenga et al.

    Recovery of megakaryocyte thromboxane production in vitro after aspirin inhibition

    Biochem. Biophys. Res. Commun.

    (1984)
  • D.J. Crutchley et al.

    Bradykinin-induced release of prostacyclin and thromboxanes from bovine artery endothelial cells. Studies with lower homologs and calcium antagonists

    Biochim. Biophys. Acta

    (1983)
  • K. Bjoro et al.

    Altered prostanoid formation in human umbilical vasculature in response to variations in oxygen tension

    Prostaglandins

    (1987)
  • C.A. Gruetter et al.

    Endothelium dependent modulation of angiotensin II induced contraction in blood vessels

    Eur. J. Pharmacol.

    (1988)
  • E. Dejana et al.

    Prostacyclin production by human endothelial and bovine smooth muscle cells in culture. Effect of repeated stimulation with arachidonic acid, thrombin, and ionophore A 23187

    Biochim. Biophys. Acta

    (1983)
  • H.L. Bush et al.

    Effect of surgical trauma on prostacyclin production by vein grafts

    Surgical Forum

    (1982)
  • S. Greenberg et al.

    Difference in prostaglandin modulation of arterial and venous muscle responses to bradykinin and norepinephrine

    Methods Find. Exper. Clin. Pharmacol.

    (1982)
  • A.J. Carter et al.

    A comparison of human pulmonary arterial and venous prostacyclin and thromboxane synthesis - effect of a thromboxane synthase inhibitor

    Thromb. Hemost.

    (1984)
  • Cited by (8)

    View all citing articles on Scopus
    View full text