Human recombinant interleukin-4 induces proliferation and interleukin-6 production by cultured human skin fibroblasts

doi:10.1016/0090-1229(92)90011-C

Clinical Immunology and Immunopathology

Volume 63, Issue 2, May 1992, Pages 182-187

Clinical Immunology ...

https://doi.org/10.1016/0090-1229(92)90011-C Get rights and content

Abstract

The effect of human recombinant interleukin-4 (hrIL-4) on normal human adult dermal fibroblasts in terms of proliferation and IL-6 production was studied. Fibroblasts were exposed to different concentrations of IL-4 for various periods of time. Proliferation was measured using a [³H]thymidine incorporation assay. IL-6 production was measured at the transcriptional, protein, and functional levels by Northern blot analysis, radioimmunoassay, and B9 bioassay, respectively. Our results show that hrIL-4 significantly stimulated (two- to fivefold) fibroblasts to increase the incorporation of [³H]thymidine in a dose- and time-dependent manner. However, hrIL-1, hrIL-2, hrIL-5, or hrTNFα, at the same concentration (100 U/ml) and for the same time period (4 days), did not. In addition, IL-4 significantly induced (four- to eightfold) the production of immunoreactive and biologically functional IL-6. However, IL-4 was not as potent an inducer of IL-6 as IL-1. The IL-4-induced IL-6 production was dose and time dependent and was due, at least in part, to a dramatic increase in the steady-state levels of IL-6 mRNA. This is the first report describing the ability of IL-4 to activate human dermal fibroblasts in terms of proliferation and IL-6 production.

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Regular articleHuman recombinant interleukin-4 induces proliferation and interleukin-6 production by cultured human skin fibroblasts

Abstract

Blood

Immunol. Today

Immunol. Today

Immunobiology

Biochem. Biophys. Res. Commun.

Clin. Immunol. Immunopathol.

Anal. Biochem.

Cell. Immunol.

Immunol. Today

Chest

Amer. J. Med. Sci.

Interleukin-6: A multifunctional cytokine regulating immune reactions and the acute phase protein response

Lab. Invest.

The interleukins

FASEB J

Interleukin-6: An overview

Annu. Rev. Immunol.

Expression and function of interleukin-6 in epithelial cells

J. Cell. Biochem.

Regulation of the acute phase and immune responses: Interleukin-6

Ann. N.Y. Acad. Sci.

Interleukin-6: Molecular pathophysiology

J. Invest. Dermatol.

Identification of a T cell-derived B cell growth factor distinet from interleukin 2

J. Exp. Med.

Interleukin-6. Historical background, genetics and biological significance

Immunol. Lett.

Characterization of the human B cell stimulatory factor 1 receptor

J. Exp. Med.

Molecular biology of interleukin 4 and interleukin 5 genes and biology of their products that stimulate B cells, T cells and hemopoietic cells

Immunol. Rev.

Interleukin-4 regulates mRNA accumulation of macrophage colony-stimulating factor by fibroblasts: Synergism with interleukin-1β

Br. J. Haematol.

Regulatory effect of recombinant interleukin (IL)3 and IL4 on cytokine gene expression of bone marrow and peripheral blood mononuclear cells

Eur. J. Immunol.

IL-4 down-regulates IL-1 and TNF gene expression in human monocytes

J. Immunol.

Regular article
Human recombinant interleukin-4 induces proliferation and interleukin-6 production by cultured human skin fibroblasts