Elsevier

Methods in Enzymology

Volume 217, 1993, Pages 618-644
Methods in Enzymology

[42] Receptor-mediated transport of DNA into eukaryotic cells

https://doi.org/10.1016/0076-6879(93)17092-JGet rights and content

Publisher Summary

This chapter describes the methods for preparing and using transferrin-polycation and other ligand-polycation conjugates for receptor-mediated DNA transfer. This method, termed “transferrinfection,” is particularly effective with cell lines derived from the erythroid lineage most likely because of the high level of cycling transferrin receptor on these cells. In other established cell lines—such as HeLa, CHO, Cos, and HepG2—this method works with efficiencies comparable to other transfection techniques. In the cells where transferrinfection functions well, the method has the following advantages over other transfection methods: (1) the method is simple to use, once the ligand-polycation conjugates are obtained, (2) the method can be used with many DNA molecules, and (3) the method is particularly gentle, involving a transferrin-polycation conjugate that the cell binds with nearly the same avidity as unmodified transferrin. Fluorescence-activated cell sorting (FACS) analyses of transfected cell populations demonstrate >95% viability at 24 hr, 48 hr, and 7 days posttransfection. This may partly account for the high efficiencies obtained.

References (60)

  • C.E. Holt et al.

    Neuron

    (1990)
  • F.L. Graham et al.

    Virology

    (1973)
  • O.-R.B. Choi et al.

    Cell

    (1988)
  • F.F. Farber et al.

    Biochim. Biophys. Acta

    (1975)
  • L.A. Lasky et al.

    Cell

    (1987)
  • J.M. Rolf et al.

    J. Biol. Chem.

    (1990)
  • E. Mattia et al.

    J. Biol. Chem.

    (1984)
  • C. Wu et al.

    J. Biol. Chem.

    (1989)
  • G.Y. Wu et al.

    J. Biol. Chem.

    (1988)
  • T. Kishimoto et al.

    Anal. Biochem.

    (1986)
  • G.L. Ellman

    Arch. Biochem. Biophys.

    (1959)
  • L. Lasky et al.

    Cell

    (1987)
  • O. Schwartz et al.

    Gene

    (1990)
  • P. Herbomel et al.

    Cell

    (1984)
  • A. Loyter et al.
  • C.A. Chen et al.

    BioTechniques

    (1988)
  • E. Neumann et al.

    EMBO J.

    (1982)
  • K. Ohtani et al.

    Nucleic Acids Res.

    (1989)
  • R. Fraley et al.

    Curr. Top. Microbiol. Immunol.

    (1982)
  • C. Nicolau, A. T. Legrand, and E. Grosse, this series, Vol. 152, p....
  • R.J. Mannino et al.

    BioTechniques

    (1988)
  • C.-Y. Wang et al.
  • P. Machy et al.
  • Y. Kanedi et al.

    Science

    (1989)
  • P.L. Felgner et al.
  • M. Graessmann et al.
  • S. Kawai et al.

    Mol. Cell. Biol.

    (1984)
  • J. McCutchan et al.

    J. Natl. Cancer Inst.

    (1968)
  • J.-P. Behr et al.
  • J.D. Appel et al.
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