[15] Immunohistochemical detection of metallothionein
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Spatial expression of metallothionein, matrix metalloproteinase-1 and Ki-67 in human epidermal wounds treated with zinc and determined by quantitative immunohistochemistry: A randomised double-blind trial
2021, European Journal of Cell BiologyCitation Excerpt :In contrast to MMP-1, MT was induced in the wound bed and in NHDFs in response to ZnSO4. The anti-MT antibody used for immunohistochemical quantification reacts with a conserved and shared N-epitope of MT1 and MT2 (Jasani and Elmes, 1991). There are 8 functional human MT1 subisoforms (MT1A, MT1B, MT1E, MT1F, MT1G, MT1H, MT1M, and MT1X) and 1 MT2 subisoform (MT2A).
Renal metallothionein responds rapidly and site specifically to zinc repletion in growing rats
2009, Journal of Trace Elements in Medicine and BiologyPrognostic significance of metallothionein in B-cell lymphomas
2006, BloodCitation Excerpt :Group comparisons were done using 2-sided, paired Student t test (equal variance assumed). Sections of formalin-fixed, paraffin-embedded biopsies were heated in a Milestone Micromed microwave oven (Sorisole, Italy) in citrate buffer (pH 6) for 15 minutes, incubated 60 minutes at room temperature with a 1:40 dilution of DAKO-MT (clone, E9; DAKO, Carpinteria, CA) reactive with MT-I and MT-II in routinely processed histologic samples,13 and stained in the Techmate 500 Immunostainer (DAKO, Glostrup, Denmark), using the DAKO Envision K5007 as a secondary antibody. Since the results suggested that some of the MT-positive cells represented macrophages, samples were also stained using CD68 (PGM1) on adjacent sections.
Evaluation of MT expression and detection of apoptotic cells in LEC rat kidneys
2004, Biochimica et Biophysica Acta - Molecular Basis of DiseaseMetallothionein in bovine spongiform encephalopathy
2002, Journal of Comparative PathologyStudy of Metallothionein Levels in the Serum of Patients with Malignant Tumour
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