Elsevier

Methods in Enzymology

Volume 98, 1983, Pages 431-444
Methods in Enzymology

[38] Recycling of insulin-sensitive glucose transporter in rat adipocytes

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Publisher Summary

This chapter discusses the recycling of insulin-sensitive glucose transporter in rat adipocytes. The chapter describes the experimental procedures that are involved in the reconstitution approach. The advantages of this approach are that the results of the study show the changes in the functional glucose transport activity and that the experiments require a considerably smaller number of animals as compared to the cytochalasin binding approach. On the other hand, the advantage of the latter approach is that the results of the experiment show the actual number of glucose transport carders. The glucose transport activity in isolated rat epididymal fat cells is stimulated by the addition of insulin, and its effect is reversed by elimination of the hormone. In addition, effects of various agents on the development and reversal of the insulin effect are tested. The treated cells are washed with an isotonic sucrose solution and homogenized. Unless otherwise specified, the indicated pH values refer to those determined at room temperature.

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