Elsevier

Human Pathology

Volume 24, Issue 8, August 1993, Pages 886-896
Human Pathology

Original contribution
KP1 (CD68)-positive large cell lymphomas: A histopathologic and immunophenotypic characterization of 12 cases

https://doi.org/10.1016/0046-8177(93)90139-8Get rights and content

Abstract

CD68KP1 antigen expression in a series of 298 non-Hodgkin's lymphoma (NHL) cases, including 41 cases of CD30Ki-1-positive anaplastic large cell (Ki-1 + ALC) lymphomas, was examined. Among the cases in this series, 12 large cell NHLs, including five centroblastic (G group according to the Working Formulation) NHLs, three immunoblastic (H group) NHLs, and four Ki-1 + ALC lymphomas, were found to express KP1. By extensive immunophenotypic analysis and in situ hybridization, KP1-positive large cell lymphomas of the G and H groups were assigned a B-cell phenotype. The pattern of KP1 staining usually consisted of localized small to medium-sized cytoplasmic dots; only two cases showed diffuse fine granular reactivity. In two of the four Ki-1 + ALC lymphomas tumor cells failed to express a B- or T-cell phenotype and stained positively for lysozyme, whereas in the other two cases they showed a hybrid T/histiocytic, phenotypic profile. KP1 staining of Ki-1 + ALC lymphoma cells was usually intense and showed a diffuse granular cytoplasmic pattern; tumor cells also expressed the CD13 antigen and showed strong reactivity with the anti-CD68 EBM11 antibody. Our results suggest that certain subsets of large “blastic” B-cell lymphomas may simultaneously express the CD68KP1 histiocyte-specific marker and other myeloid-associated antigens, indicating the necessity of using a multiparameter approach in the determination of cell lineage. Moreover, this study, which demonstrates that the expression of CD68KP1 and CD30 antigens is not mutually exclusive, supports the view that a fraction of cases diagnosed as Ki-1 + ALC lymphomas (at least those with KP1 expression along with the lack of B- or T-antigen expression) represent true histiocytic lymphomas despite the Ki-1 + phenotype.

References (53)

  • LM Weiss et al.

    Large-cell hematolymphoid neoplasms of uncertain lineage

    Hum Pathol

    (1988)
  • KAF Pulford et al.

    KP1: A new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections

    J Clin Pathol

    (1989)
  • RA Warnke et al.

    Diagnosis of myelomonocytic and macrophage neoplasms in routinely processed tissue biopsies with monoclonal antibody KP1

    Am J Pathol

    (1989)
  • F Facchetti et al.

    KP1 (CD68) staining of malignant melanomas

    Histopathology

    (1991)
  • A Gloghini et al.

    Malignant fibrous histiocytoma

    Am J Clin Pathol

    (1993)
  • SW Binder et al.

    A histiocyte-specific marker in the diagnosis of malignant fibrous histiocytoma. Use of monoclonal antibody KP-1 (CD68)

    Am J Clin Pathol

    (1992)
  • The Non-Hodgkin's Lymphoma Pathologic Classification Project

    National Cancer Institute study of classification of non-Hodgkin's lymphomas. Summary and description of a Working Formulation for clinical usage

    Cancer

    (1982)
  • BA Agnarsson et al.

    Ki-1 positive large cell lymphoma. A morphologic and immunologic study of 19 cases

    Am J Surg Pathol

    (1988)
  • CA Hanson et al.

    True histiocytic lymphoma: Histopathologic, immunophenotypic and genotypic analysis

    Br J Haematol

    (1989)
  • A Carbone et al.

    Anti-vimentin antibody reactivity with Reed-Sternberg cells of Hodgkin's disease

    Virchows Arch A Pathol Anat Histopathol

    (1990)
  • A Carbone et al.

    Heterogeneous in situ immunophenotyping of follicular dendritic reticulum cells in malignant lymphomas of B-cell origin

    Cancer

    (1987)
  • A Carbone et al.

    Expression of Leu-8 surface antigen in B-cell lymphomas. Correlation with other B-cell markers

    J Pathol

    (1988)
  • A Carbone et al.

    Histopathologic, immunophenotypic, and genotypic analysis of Ki-1 anaplastic large cell lymphomas that express histiocyte-associated antigens

    Cancer

    (1990)
  • A Carbone et al.

    Report of an unusual small lymphocytic B-cell lymphoma selectively involving the B-zone of lymph node

    Cancer

    (1990)
  • S Carrell et al.

    Expression on human thymocytes of the idiotypic structures (Ti) from two leukemia T cell lines Jurkat and HPB-ALL

    Eur J Immunol

    (1986)
  • Y Choi et al.

    A method for production of antibodies to human T-cell receptor β-chain variable regions

  • Cited by (0)

    Supported in part by the Associazione Italiana per la Ricerca sul Cancro, Milan, Italy; by the Consiglio Nazionale delle Ricerche, PF-ACRO; and by the Ministero della Sanità, Ricerca Finalizzata IRCCS 1990 and 1991, Rome, Italy.

    View full text