Elsevier

Aquaculture

Volume 50, Issues 1–2, 15 November 1985, Pages 75-87
Aquaculture

Quantitative methionine requirement of juvenile sea-bass (Dicentrarchus labrax)

https://doi.org/10.1016/0044-8486(85)90154-1Get rights and content

Abstract

The methionine (MET) requirement of sea-bass was studied by the nutrient-response relationship using growth rate data and free MET levels in serum and whole blood after feeding. Two series of diets were employed, sharing a common basal diet. This basal diet contained a mixture of soybean, yeast and fish meals as protein source, and was formulated to be deficient in MET whereas the other essential amino acids levels corresponded to the pattern found in sea-bass muscle proteins. Graded levels of MET were obtained by supplementing the basal diet with crystalline MET (S series) or by substituting soybean and yeast meals by fish meal (F series). Fish were fed for 85 days on an optimal ration (1.5%/day). The MET requirement could be determined only with the F series diets using sigmoidal mathematical models. Inflexion points from curves obtained with the data for weight gain and for free MET in the blood were very similar. The MET requirement was estimated at about 1% of dry diet or 2% of dietary protein or 15 mg/day per 100 g live weight. The S series diets allowed us to determine, from growth data, the optimal level of MET supplementation: optimal growth rate occurred with 1.3% total MET content in the diet, with 0.6% in the crystalline form. Further supplementation depressed growth rate. Choices of types of diet, advantages of the biochemical method and use of sigmoidal models for computer fitting of experimental data are discussed.

References (40)

  • K.R. Robbins et al.

    Histidine status in the chick as measured by growth rate, plasma free histidine and breast muscle carnosine

    J. Nutr.

    (1977)
  • K.R. Robbins et al.

    Estimation of nutrient requirement from growth data

    J. Nutr.

    (1979)
  • H. Thébault

    Plasma essential amino acid changes in sea-bass (Dicentrarchus labrax) after feeding diets deficient and supplemented in L-methionine

    Comp. Biochem. Physiol.

    (1985)
  • S. Viola et al.

    Effects of soybean processing methods on the growth of carp (Cyprinus carpio)

    Aquaculture

    (1983)
  • M.J. Walton et al.

    Methionine metabolism in rainbow trout fed diets of differing methionine and cystine content

    J. Nutr.

    (1982)
  • E. Alliot et al.

    Besoins nutritifs du bar (Dicentrarchus labrax). Etude du taux de protéines et du taux de lipides dans le régime

  • E. Alliot et al.

    Etude de l'apport calorico-azoté dans l'alimentation du bar (Dicentrarchus labrax). Influence sur la croissance et la composition corporelle

  • E. Alliot et al.

    Amélioration des formules d'aliments artificiels pour le loup, Dicentrarchus labrax. I. Etude préliminaire du besoin quantitatif en Méthionine

  • A.H. Coleman

    Plasma versus red blood cells lysine levels as an indicator for protein nutritional status

  • C.B. Cowey

    Protein and amino acid requirements of finfish

  • Cited by (50)

    • Optimum dietary methionine requirement of juvenile silver pompano, Trachinotus blochii (Lacepede, 1801)

      2020, Animal Feed Science and Technology
      Citation Excerpt :

      The optimum methionine requirement for silver pompano ranged from 11.6–11.8 g/kg diet that corresponded to 27.6–28.1 g/kg CP based on the quadratic regression analysis of the PER, feed utilization, and growth data. These results are similar to the dietary methionine requisite of fish species such as juvenile Trachinotus ovatus (24.6–29.5 g/kg CP; Niu et al., 2013), juvenile Sebastes schlegelii (28.0 g/kg CP; Yan et al., 2007), juvenile Rachycentron canadum (26.4 g/kg CP; Zhou et al., 2006), juvenile Epinephelus coioides (27.3 g/kg CP; Luo et al., 2005), Asian sea bass (29.0 g/kg CP; Coloso et al., 1999), yellowtail (26.0 g/kg CP; Ruchimat et al., 1997), coho salmon (27.0 g/kg CP; Arai and Ogata, 1993), and rohu (26.0 g/kg CP; Khan and Jafri, 1993) but lower than that reported for Pseudosciaena crocea R. (33.4 g/kg CP; Mai et al., 2006), Indian major carp (Cirrhinus mrigala) (30.0 g/kg CP; Ahmed et al., 2003), Paralichthys olivaceus (31.0 g/kg CP; Alam et al., 2001), Atlantic salmon (31.0 g/kg CP; Rollin, 1999), Indian major carp (Labeo rohita Hamilton) (32.0 g/kg CP; Murthy and Varghese, 1998), African catfish (32.0 g/kg CP; Fagbenro et al., 1998), catla (36.0 g/kg CP; Ravi and Devaraj, 1991), red drum (30.0 g/kg CP; Moon and Gatlin, 1991), and common carp (31.0 g/kg CP; Nose, 1979) and higher than that reported for Japanese flounder (19.0 g/kg CP; Alam et al., 2001), Arctic charr (18.0 g/kg CP; Simmons et al., 1999), rainbow trout (23.0 g/kg CP; Kim et al., 1992; Rodehutscord et al., 1995), milkfish (25.0 g/kg CP; Borlongan and Coloso, 1993), Hybrid striped bass (Morone chrysops x Morone saxatilis) 18−20 g/kg of CP (Keembiyehetty and Gatlin, 1993) and European sea bass (20.0 g/kg CP; Thebault et al., 1985). In general, carnivorous fish varieties require higher levels of protein in their diet and demand higher dietary methionine than omnivores and herbivores.

    • Effects of dietary methionine and taurine supplementation to low-fish meal diets on growth performance and oxidative status of European sea bass (Dicentrarchus labrax) juveniles

      2017, Aquaculture
      Citation Excerpt :

      All fish as other animals are recognised to have a dietary need for both Met and total sulphur AA (TSAA; Met + Cys) (Wilson, 2002). For European sea bass, Met requirement for maximum growth was estimated to be 1.3% (35 g fish; Thebault et al., 1985) or 0.9% of the diet (Hidalgo et al., 1987), corresponding to a TSAA level of 1.9 to 2.2%, respectively. More recently, Tulli et al. (2010) estimated Met requirement to be 0.8–0.9% of the diet and TSAA requirement to be 1.2–1.3% of the diet (13 g European sea bass), and observed no depression of growth or N retention at the highest Met and TSAA levels tested (1.6% and 2.0% of the diet, respectively).

    • Total sulfur amino acid requirement and cystine replacement value for fingerling stinging catfish, Heteropneustes fossilis (Bloch)

      2014, Aquaculture
      Citation Excerpt :

      Fingerling H. fossilis fed the methionine deficient diets did not exhibit any lens opacity. Similarly, Thebault et al. (1985), Hidalgo et al. (1987) in their studies on sulfur amino acid nutrition of sea bass, Abidi and Khan (2011) in Rohu and Khan and Abidi (2011c) in Mrigal did not observe cataract when fed diets deficient in methionine. Flesh quality is of increasing concern to the aquaculture industry.

    View all citing articles on Scopus
    View full text