Elsevier

Virology

Volume 178, Issue 1, September 1990, Pages 52-61
Virology

Regular article
Immunodetection in Vivo of beet necrotic yellow vein virus-encoded proteins

https://doi.org/10.1016/0042-6822(90)90378-5Get rights and content

Abstract

Open reading frames identified on the four genomic RNAs of beet necrotic yellow vein virus were cloned into bacterial expression vectors and resulting cl-fusion proteins expressed in Escherichia coli were used to raise polyclonal antibodies. This set of antisera was used to show the presence of 7 of 9 predicted viral proteins in mechanically inoculated Chenopodium quinoa leaves by the Western blot technique. Viral coat protein (p22) and its readthrough protein p85 encoded by RNA-2 could be detected in all subcellular fractions. Two other RNA-2-encoded proteins, p42 and p13, are predominantly associated with membranous structures. Another RNA-2-encoded protein, pt 4, as well as the two polypeptides p25 and p31, encoded by RNA-3 and -4, respectively, are soluble proteins. The viral proteins could first be detected about the time lesions became visible and increased thereafter except for p85, in which case the amount of the soluble form decreased with time. No protein could be detected corresponding to the RNA-1-encoded p237 protein or to the pt 5 species encoded by open reading frame V of RNA-2.

References (43)

  • R.L.S. Forster et al.

    The complete nucleotide sequence of the potexvirus white clover mosaic virus

    Nucleic Acids Res.

    (1988)
  • T. Godefroy-Colburn et al.

    A non-structural protein of alfalfa mosaic virus in the walls of infected tobacco cells

    J. Gen. Virol.

    (1986)
  • R. Goldbach

    Genome similarities between plant and animal RNA viruses

    Microbiol. Sci.

    (1987)
  • A.E. Gorbalenya et al.

    A conserved NTP-motif in putative helicases

    Nature (London)

    (1988)
  • T.C. Hodgman

    A new superfamily of replicative proteins

    Nature (London)

    (1988)

    Nature (London)

    (1988)
  • I. Jupin et al.

    In vitro translation of natural and synthetic beet necrotic yellow vein virus RNA-1

    J. Gen. virol.

    (1988)
  • R. Klug et al.

    “Zinc Fingers”: A novel protein motif for nucleic acid recognition

    Trends Biochem. Sci.

    (1987)
  • R. König et al.

    Uniform RNA patterns of beet necrotic yellow vein virus in sugarbeet roots but not in leaves from several plant species

    J. Gen. Virol.

    (1986)
  • R. Kornfeld et al.

    Assembly of asparaginelinked oligosaccharides

    Annu. Rev. Biochem.

    (1985)
  • M. Kuszala et al.

    Beet necrotic yellow vein virus: Different isolates are serologically similar but differ in RNA composition

    Ann. Appl. Biol.

    (1986)
  • U.K. Laemmli

    Cleavage of structural proteins during the assembly of the head of bacteriophage T4

    Nature (London)

    (1970)
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