Elsevier

Virology

Volume 65, Issue 2, June 1975, Pages 436-445
Virology

Studies on the replication of reticuloendotheliosis virus: Detection of viral-specific DNA sequences in infected chick cells

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Abstract

Infection of stationary chick embryo fibroblasts by reticuloendotheliosis virus (REV) is sensitive to cytosine arabinoside, an inhibitor of DNA synthesis. Furthermore, the majority of the nucleic acid sequences contained in the REV genome can be detected in infected cells in the form of DNA by RNA-DNA hybridization techniques. A small portion (ca. 5%) of the REV-specific sequences can be detected in uninfected chick embryo fibroblasts suggesting that these cells contain at least part of the REV genome as endogenous DNA sequences. These observations are consistent with an involvement of REV-specific proviral DNA as an intermediate in the replication of REV in chick embryo fibroblasts.

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  • Cited by (16)

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    1

    Present address: Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455.

    2

    Present address: Department of Biochemistry, University of Pittsburgh, Pittsburgh, PA 15261.

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