Elsevier

Talanta

Volume 19, Issue 6, June 1972, Pages 769-778
Talanta

Electrochemical determination of adenine and adenosine: Adsorption of adenine and adenosine at the pyrolytic graphite electrode

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Abstract

Adenine and adenosine are polarographically reducible from aqueous solution at pH 4.7 at the DME at the same E12 and are also voltammetrically oxidizable at the PGE, but at different potentials, adenosine at higher potentials. Competitive adsorption of both compounds at the PGE results in a decrease in the scanning voltammetric oxidation peak of adenine in the presence of adenosine, reaching a constant value when the concentration of the latter is above 6 mM. In mixtures, the sum of the two is obtained by polarography at the DME. Solid adenosine is then added to the solution and the adenine is determined by voltamrnetry at the PGE.

Zusammenfassung

Adenin und Adenosin lassen sich aus wäßriger Lösung bei pH 4.7 polarographisch an der Quecksilbertropfelektrode (DME) beim selben Halbstufenpotential reduzieren. An einer Elektrode aus Pyrolysegraphit (POE) sind beide voltammetrisch oxidierbar, Adenosin jedoch bei höherem Potential. Die konkurrierende Adsorption beider Verbindungen an der PGE führt dazu, daß der Adeninpeak beim Registrieren der voltammetrischen Oxidation in Gegenwart von Adenosin kleiner wird und bei einer Adenosinkonzentration über 6 mM einen konstanten Wert erreicht. In Gemischen wird die Summe beider Verbindungen durch Polarographie an der DME erhalten. Dann wird festes Adenosin zur Lösung gegeben und Adenin durch Voltammetrie an der PGE bestimmt.

Résumé

Ládénine et l'adénosine sont réductibles polarographiquement en solution aqueuse à pH 4,7 à l'électrode à goutte de mercure au même E12, et sont également oxydables voltammétriquement à l'élec trode de toile de platine, mais à des potentiels différents, l'adénosine aux potentiels plus élevés. L'adsorption compétitive des deux composés sur l'électrode de toile de platine a pour conséquence un abaissement dans le pic d'oxydation voltammétrique de l'adénine en la présence d'adénosine, atteignant une valeur constante lorsque la concentration de cette dernière est supérieure à 6 mM. Dans les mélanges, la somme des deux est obtenue par polarographie à l'électrode à goutte de mercure. On ajoute alors de l'adénosine solide à la solution et l'on dose l'adénine par voltammétrie a l'électrode de toile de platine.

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