Elsevier

Steroids

Volume 34, Issue 1, July 1979, Pages 73-88
Steroids

Bile acid sulfates in serum bile acids determination

https://doi.org/10.1016/0039-128X(79)90127-2Get rights and content

Abstract

Some bile acid sulfates were synthesized and characterized. The configuration of sulfate groups at C-3, C-7 and C-12 positions was confirmed by Nuclear Magnetic Resonance analysis. These sulfates were utilized in a study of their chemical behaviour in different analytical procedures currently used for serum bile acids determination. Procedures for bile acids extraction from serum with ethanol or Anberlite XAD-2 result in an important loss of the most polar sulfated bile acids. Complete separation of unsulfated from sulfated bile acids on Sephadex LH-20 is not achieved when deconjugation has not been previously performed. Alkaline hydrolysis of some sulfated bile acids induces artifacts. Enzymatic deconjugation of the most polar bile acid sulfate is slow but does not produce artifacts. Enzymatic determination of bile acids gives positive response with some bile acid sulfates. The current procedures of serum bile acids determination are discussed in consideration of these results.

References (27)

  • I. Makino et al.

    J. Lipid. Res.

    (1974)
  • C.B. Campbell et al.

    Clin. Chim. Acta

    (1975)
  • I. Makino et al.

    Gastroenteroloty

    (1975)
  • H.P. Schwarz et al.

    Clin. Chim. Acta

    (1974)
  • G. Parmentier et al.

    J. Chromatog.

    (1978)
  • J.F. Pageaux et al.

    Clin. Chim. Acta

    (1978)
  • G. Parmentier et al.

    Steroids

    (1975)
  • G.P. Van Berge Henegouwen et al.

    J. Lipid. Res.

    (1977)
  • K.Y. Tserng et al.

    J. Lipid. Res.

    (1977)
  • G. Parmentier et al.

    Steroids

    (1977)
  • G.P. Van Berge Henegouwen et al.

    Clin. Chim. Acta

    (1976)
  • B. Alme et al.

    J. Lipid. Res.

    (1977)
  • H.L. Bradlow

    Steroids

    (1977)
  • Cited by (19)

    • Overcoming environmental problems of biocides: Synthetic bile acid derivatives as a sustainable alternative

      2020, Ecotoxicology and Environmental Safety
      Citation Excerpt :

      Sulfated derivatives were obtained according to Correia-da-Silva et al. (2011a, 2011b, 2011c). The use of microwave irradiation in sulfation of bile acid 1, allowed to obtain the sulfated derivative in shorter reaction times and higher yield than previously described (Pageaux et al., 1979). N-Deoxycholylethylamine (7) was obtained by refluxing compound 5 in ethylenediamine according to Lee et al. (2007), in 97% yield (Scheme 1 A).

    • Bile acids

      1986, Advances in Clinical Chemistry
    View all citing articles on Scopus
    View full text