Influence of methionine on the mutation frequency in Salmonella typhimurium

doi:10.1016/0027-5107(82)90295-0

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis

Volume 94, Issue 2, June 1982, Pages 331-338

https://doi.org/10.1016/0027-5107(82)90295-0 Get rights and content

Abstract

When reversion of some markers in S. typhimurium was tested, it was found that addition of methionine to test plates increased the revertant yields. Two assumptions were made: (1) the effect of methionine reflects the necessity of intensive synthesis of some protein(s) required for abolishing the deleterious effects and/or expressing the mutagenic effects induced by mutagens; (2) methionine influences the mutagenesis via the DNA methylation process.

References (16)

D. Anton
Histidine regulatory mutants in Salmonella typhimurium, V. Two new classes of histidine regulatory mutants
J. Mol. Biol.
(1968)
G.R. Fink et al.
Histidine regulatory mutants in Salmonella typhimurium, VI. Dominance studies
J. Mol. Biol.
(1968)
T.S. Gross et al.
Methionyl-transfer RNA synthetase mutants of Salmonella typhimurium which have normal control of the methionine biosynthesis enzymes
Biochim. Biophys. Acta
(1969)
P.E. Hartman et al.
Classification and mapping of spontaneous and induced mutation in the histidine operon of Salmonella
Adv. Genet.
(1971)
C. Janion
The efficiency and extent of mutagenic activity of some new mutagens of base analogue type
Mutation Res.
(1978)
r.F. Kimball
The relation of repair phenomena to mutation in bacteria
Mutation Res.
(1978)
H.L. Vogel et al.
Acetylonitrase of Escherichia coli, partial purification and some properties
J. Biol. Chem.
(1956)
B.N. Ames
The detection of chemicals mutagens with enterio bacteria

There are more references available in the full text version of this article.

Cited by (5)

Mutagenesis and anti-mutagenesis in Salmonella: influence of ethionine and caffeine on yields of mutations induced by 2-aminopurine and 9-aminoacridine
1983, Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Ethionine, the ethyl analogue of methionine, slightly reduced the yield of reversions of the hisC3076 frameshift marker induced by 9-aminoacridine (9AA) in an excision-proficient strain of Salmonella typhimurium, but completely abolished mutagenesis genesis by 9AA in the excision-deficient uvrB-deletion strain TA1537. No toxic effects of ethionine were apparent in either the excision-proficient or the excision-deficient strain. Because of the differential effects of ethionine on mutagenesis in the two strains, it seemed possible that an ethionine-sensitive step in the process(es) leading to fixation of 9AA-induced mutations might be compensated for by the uvrA,B,C⁺ excision-repair system. To further test this possibility, we used caffeine (a compound known to significantly reduce the efficacy of the excision-repair process) as a co-treatment with ethionine for cells of an excision-proficient strain exposed to 9AA. Treatment with caffeine alone or ethionine alone had very little effect on reversion yield, whereas co-treatment with the two agents abolished 9AA mutagenesis. It appeared, therefore, that either the caffeine-sensitive pathway or the ethionine-sensitive pathway needed to be functioning if 9AA-induced reversions of the hisC3076 marker were to be detected. Addition of methionine to cells of the excision-deficient strain exposed to 9AA restored their ability to be mutated by 9AA, however. In a base-pair substitution back-mutation system, ethionine slightly enhanced the yields of revertants of the trpE8 marker induced by 2-aminopurine (2AP) in both an excision-proficient strain (at all 2AP dose levels tested) and an excision-deficient strain (only at the lower dose levels). In the excision-deficient strain, doses of 2AP above 300 μg/plate were highly toxic when ethionine was also present. It was for this reason that no 2AP-induced revertants were recovered at the higher 2AP concentrations. Treatment of the trpE8 strain with methionine also enhanced the yield of 2AP-induced revertants of this marker.
An Aptitude for Altitude: Are Epigenomic Processes Involved?
2019, Frontiers in Physiology
Epigenomics and human adaptation to high altitude
2017, Journal of Applied Physiology
Bridging sequence diversity and tissue-specific expression by DNA methylation in genes of the mouse prolactin superfamily
2012, Mammalian Genome
Stochastic epigenetic variation as a driving force of development, evolutionary adaptation, and disease
2010, Proceedings of the National Academy of Sciences of the United States of America

View full text

Article preview

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis

Abstract

References (16)

Histidine regulatory mutants in Salmonella typhimurium, V. Two new classes of histidine regulatory mutants

J. Mol. Biol.

Histidine regulatory mutants in Salmonella typhimurium, VI. Dominance studies

J. Mol. Biol.

Methionyl-transfer RNA synthetase mutants of Salmonella typhimurium which have normal control of the methionine biosynthesis enzymes

Biochim. Biophys. Acta

Classification and mapping of spontaneous and induced mutation in the histidine operon of Salmonella

Adv. Genet.

The efficiency and extent of mutagenic activity of some new mutagens of base analogue type

Mutation Res.

The relation of repair phenomena to mutation in bacteria

Mutation Res.

Acetylonitrase of Escherichia coli, partial purification and some properties

J. Biol. Chem.

The detection of chemicals mutagens with enterio bacteria

Cited by (5)

Mutagenesis and anti-mutagenesis in Salmonella: influence of ethionine and caffeine on yields of mutations induced by 2-aminopurine and 9-aminoacridine

An Aptitude for Altitude: Are Epigenomic Processes Involved?

Epigenomics and human adaptation to high altitude

Bridging sequence diversity and tissue-specific expression by DNA methylation in genes of the mouse prolactin superfamily

Stochastic epigenetic variation as a driving force of development, evolutionary adaptation, and disease