Sample handling techniques when analyzing regulatory peptides
References (7)
- et al.
Regulatory Peptides
(1984) - et al.
Peptides
(1986) - et al.
Regulatory Peptides
(1983)
Cited by (81)
The impact of biosampling procedures on molecular data interpretation
2013, Molecular and Cellular ProteomicsCitation Excerpt :Their abundance is dependent on the severity of the infarction. Examples of commonly used biomarkers requiring short BTIs are glucagon-like peptide-1 (GLP-1), gastric inhibitory polypeptide, glucagon, and ghrelin (27). In addition, the complement activation and coagulation pathways present in blood introduce a specific set of protease-associated bioreactions, influencing protein degradation patterns during the BTI (15).
The anorectic response to growth hormone in obese rats is associated with an increased rate of lipid oxidation and decreased hypothalamic galanin
2011, Physiology and BehaviorCitation Excerpt :The RER was calculated as the ratio between the CO2 produced and the O2 produced (ΔCO2/ΔO2) for each animal, and the EE as the product of the calorific value of oxygen (CV) and ΔO2 per kg0.67 of body weight (CV = 3.815 + 1.232 * RER). The extraction of hypothalamic galanin and NPY was performed as described previously [32]. In short, the frozen hypothalamic samples were first cut into small pieces.
Alteration of Neuropeptides in the Lung Tissue Correlates Brain Death-Induced Neurogenic Edema
2009, Journal of Heart and Lung TransplantationCitation Excerpt :The supernatants were lyophilized and stored at −70°C until neuropeptide analysis. Plasma samples were extracted before analysis on C18-reverse-phase cartridges, as described elsewhere.23 Transthoracic echocardiography was performed 6 times: at baseline, at the time of brain death, and 30, 60, 120, and 360 minutes after brain death using a Vivid 7 ultrasound machine equipped with a 2.5-MHz matrix probe (GE Healthcare, Horten, Norway).
Pharmacokinetic profile of orexin A and effects on plasma insulin and glucagon in the rat
2004, Regulatory PeptidesMicrodialysis of neuropeptide Y in human muscle tissue
2004, Journal of Neuroscience MethodsExtraction and radioimmunoassay quantitation of neuropeptide Y (NPY) and vasoactive intestinal polypeptide (VIP) from human dental pulp tissue
2003, Archives of Oral BiologyCitation Excerpt :The fraction numbers containing NPY and VIP immunoreactivity in the pulp extract (extracted by boiling in acetic acid) closely matched the retention times of synthetic peptides indicating authentic NPY and VIP extraction from the dental pulp sample. In general organic solvent extraction has been recommended for extraction of neuropeptides from plasma,16 and gastrointestinal tissues,13 while acidic solvent extraction has been recommended for tissues with gelatinous components.13 Since the dental pulp tissue has a large gelatinous component, acidic solvent extraction should yield higher concentration of neuropeptides than organic solvent extraction.