Elsevier

Life Sciences

Volume 31, Issue 19, 8 November 1982, Pages 2093-2103
Life Sciences

Prostaglandin D2 in rat brain, spinal cord and pituitary: Basal level and regional distribution

https://doi.org/10.1016/0024-3205(82)90101-1Get rights and content

Abstract

A highly sensitive and specific radioimmunoassay for prostaglandin D2 has been developed and used to determine the basal level and regional distribution of this prostaglandin in rat brain, spinal cord and pituitary. The assay can detect as little as 20 pg of prostaglandin D2, and the antiserum used shows 20% cross-reactivity to prostaglandin D1, 0.1% cross-reactivity to 13,14-dihydro-15-ketoprostaglandin D2 and even lower cross-reactivity to other prostaglandins. Prostaglandin D2-like immunoreactivity was extracted with ethanol from the rat tissues. The immunoreactivity comigrated with authentic prostaglandin D2 on silica gel thin layer chromatography, showed the dilution curve parallel to that of the authentic compound, and decreased in amounts by the pretreatment of animals with indomethacin, suggesting that it was prostaglandin D2 itself. To avoid a postmortem formation of prostaglandins, we sacrificed animals by microwave irradiation at 4.5 kW for 1.2 sec under which conditions both prostaglandin D synthetase and prostaglandin D dehydrogenase were completely inactivated. The amount of prostaglandin D2 in whole brain measured under these conditions was 3.42±0.59 ng (mean+S.E.M.), and those of prostaglandin E2 and F measured by the respective radioimmunoassays were 1.32±0.24 and 0.96±0.20 ng, respectively. Prostaglandin D2 was widely distributed in rat brain, spinal cord and pituitary. The highest concentrations were seen in pineal gland and neurointermediate pituitary followed by anterior pituitary. Lower but significant concentrations were observed in other parts of brain, among which hypothalamus and septum showed the relatively high concentrations.

References (37)

  • M.S. Abdel-Halim et al.

    Prostaglandins

    (1977)
  • F.F. Sun et al.

    Prostaglandins

    (1977)
  • M.S. Abdel-Halim et al.

    Prostaglandins

    (1979)
  • M.S. Abdel-Halim et al.

    Prostaglandins

    (1980)
  • E. Berchtold-Kanz et al.

    Prostaglandins

    (1981)
  • I. Bishai et al.

    Biochim. Biophys. Acta

    (1981)
  • T. Shimizu et al.

    J. Biol. Chem.

    (1979)
  • K. Watanabe et al.

    J. Biol. Chem.

    (1980)
  • K. Kondo et al.

    Biochem. Biophys. Res. Commun.

    (1981)
  • R.J. Miller et al.

    J. Biol. Chem.

    (1978)
  • W.S. Powell

    Prostaglandins

    (1980)
  • T. Watanabe et al.

    Arch. Biochem. Biophys.

    (1982)
  • Y. Maruyama

    Trends in Pharmacol. Sci.

    (1981)
  • J. Nakano et al.

    Brain Res.

    (1972)
  • N.G. Bazán

    Biochim. Biophys. Acta

    (1970)
  • E. Bosisio et al.

    Prostaglandins

    (1976)
  • S.R. Ojeda et al.

    Brain Res.

    (1978)
  • G. Cseh et al.

    Brain Res. Bull.

    (1978)
  • Cited by (175)

    • Adenosinergic Control of Sleep/Wake Behavior

      2019, Handbook of Behavioral Neuroscience
    • Neurobiological Basis of Hypersomnia

      2017, Sleep Medicine Clinics
    • Interleukin-1β induces sleep independent of prostaglandin D<inf>2</inf> in rats and mice

      2017, Neuroscience
      Citation Excerpt :

      PGH2 is immediately converted by terminal PG synthases to PGD2, PGE2, PGF2α, PGI2, and thromboxane A2. The most abundant prostanoid in the brains of rats and other mammals is PGD2 (Narumiya et al., 1982). PGD2 is produced by lipocalin-type PGD synthase (L-PGDS), which is localized mainly in the leptomeninges, choroid plexus, and oligodendrocytes in the brain (Narumiya et al., 1982; Urade et al., 1993).

    • Roles of adenosine and its receptors in sleep-wake regulation

      2014, International Review of Neurobiology
    View all citing articles on Scopus

    This work was supported in part by a Grant-in Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan, and grants from the Japanese Foundation on Metabolism and Diseases, Fujiwara Memorial Foundation, Japan Heart Foundation, and the Wellcome Foundation.

    View full text