Liver adenine nucleotide metabolism during hypothermic anoxia and a recovery period in perfusion

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Abstract

Reduction of the level of ATP and of the total adenine nucleotide pool has been studied during cold storage of livers initially flushed with Ringer's lactate (R-L), Collins' solution (CO), modified Collins' solution (CMg), and a new hyperosmolar “intracellular” solution containing sucrose (KMgS).

The reduction of ATP and nucleotides after 24 hr of anoxia is not influenced by the composition of the flushing solution. When the preserved livers are reperfused with oxygenated blood, the remaining adenine nucleotides are rapidly rephosphorylated, attesting to the persistence of mitochondrial functions irrespective of the solution used. In contrast, a synthesis of adenine nucleotides during the first hours following the anoxic period can only be obtained with the two solutions which have the highest osmolarity and magnesium sulfate content, the CMg and KMgS solutions.

References (30)

  • H.L. Segal et al.

    5′-Nucleotidase of rat liver microsomes

    J. Biol. Chem.

    (1960)
  • W.D. Yushok

    Control mechanisms of adenine nucleotides metabolism of ascites tumor cells

    J. Biol. Chem.

    (1971)
  • H. Acquatella et al.

    Ionic and histological changes in the kidney after perfusion and storage for transplantation

  • M.B. Burg et al.

    Active cation transport by kidney tubules at 0°C

    Amer. J. Physiol.

    (1964)
  • M.R. Buhl et al.

    Breakdown of 5′ adenine nucleotides in ischemic renal cortex estimated by oxypurine excretion during perfusion

    Scand. J. Clin. Lab. Invest.

    (1975)
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