Journal of Molecular Biology
Volume 89, Issue 3, 5 November 1974, Pages 469-472, IN7-IN8, 473-475
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Location of a platinum binding site in the structure of yeast phenylalanine transfer RNA

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Abstract

Trans-dichlorodiammineplatinum (II) reacts with yeast phenylalanine transfer RNA to yield a major platinum binding site. The tightly bound platinum has been located on the oligonucleotide Gm-A-A-Y-A-ψp containing the anticodon by standard fingerprinting methods using 32P-labelled tRNAPhe. This site corresponds to a single major platinum site identified during an X-ray crystallographic analysis of yeast tRNAPhe. The solution studies have given confidence to the assignment of part of the 3 Å electron density map to the anticodon region of the molecular structure of yeast tRNAPhe.

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One of us (P. W. P.) was supported by an M.R.C. Scholarship for Training in Research Methods.

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