Journal of Molecular Biology
Volume 38, Issue 2, 14 December 1968, Pages 217-225
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Replication of bacteriophage DNA: I. Replication of DNA of lambda phage defective in early functions

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Abstract

DNA synthesis of early sus (suppressor sensitive) mutants of phage λ in su cells was studied by the density-gradient centrifugation method. The functions of the O and P cistrons are essential for replication of the phage genome while the function of the N cistron is not. DNA molecules of susN mutants replicate at a slow rate. A majority of the molecules replicate more than once. For normal replication to occur the N function has to be expressed.

The functions of the O and P cistrons are expressed in the absence of the N function. Superinfection of a heteroimmune lysogen by a susP mutant fails to elicit the function required for replication of the superinfecting phage genome.

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This work was aided in part research grant GM-08384 from the United States Public Health Service.

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