Aspects of substrate utilization and energy requirement during molluscan phagocytosis

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Abstract

Unlike the case of mammalian phagocytes, there is no increase in oxygen consumption by hemolymph cells of Mercenaria mercenaria actively phagocytosing Bacillus megaterium.

The utilization of glucose and glycogen, coupled with the production of lactate and no increase in oxygen utilization, by M. mercenaria cells actively engaged in phagocytosis indicates that glycolysis is the energy-providing pathway. This conclusion is strengthened by the fact that KCN does not inhibit phagocytosis.

Nitroblue tetrazolium reduction characteristic of mammalian phagocytes is absent in M. mercenaria hemolymph cells.

The myeloperoxidase-H2O2-halide antimicrobial system of mammalian phagocytes is absent in hemolymph cells of M. mercenaria.

References (28)

  • T.C. Cheng et al.

    An electron microscope study of the fate of bacteria phagocytized by granulocytes of Crassostrea virginica

    Contemp. Top. Immunobiol

    (1974)
  • T.C. Cheng et al.

    Cellular reactions in marine pelecypods as a factor influencing endosymbioses

  • Z.A. Cohen et al.

    Functional and metabolic properties of polymorphonuclear leukocytes. I. Observations on the requirements and consequences of particle ingestion

    J. Exp. Med

    (1960)
  • B. Holmes et al.

    Studies of the metabolic activity of leukocytes from patients with a genetic abnormality of phagocytic function

    J. Clin. Invest

    (1967)
  • Cited by (0)

    This research was supported by a grant (No. FD-00416-05) from the U.S. Public Health Service.

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