Purification and properties of sorbitol dehydrogenase from mouse liver

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Abstract

  • 1.

    1. The sorbitol dehydrogenase (l-iditol: NAD oxidoreductase, EC 1.1.1.14) from mouse liver has been purified to homogeneity.

  • 2.

    2. The enzyme has a mol. wt of 140,000 and is composed of four identical subunits of mol. wt 35,000.

  • 3.

    3. The purified enzyme catalyses both sorbitol oxidation and fructose reduction.

  • 4.

    4. It is specific for NAD+ (NADH) and does not function with NADP+ (NADPH).

  • 5.

    5. The Michaelis constants for sorbitol, fructose, NAD++ and NADPH are 1.54 and 154 mM, 58.8 and 15 μM, respectively.

  • 6.

    6. The enzyme is SH-group reagent sensitive and is strongly inhibited by 1,10-phenanthroline.

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