Elsevier

Immunochemistry

Volume 7, Issue 5, May 1970, Pages 413-422
Immunochemistry

The effect of borate on polysaccharide-protein and antigen-antibody reactions and its use for the purification and fractionation of crossreacting antibodies

https://doi.org/10.1016/0019-2791(70)90223-5Get rights and content

Abstract

Borate has been shown to inhibit precipatation between anti carbohydrate antibodies, concanavalin A and Helix pomatia A hemagglutinin and various polysaccharides. The inhibitory effect was most pronounced in systems in which the association constant for the sytems is low. A crossreacting horse anti type II pneumococcal polysaccharidedextran B 512 precipitate could be solubilized and the antibodies separated free from the by gel-filtration. Consecutive borate extractions of the precipitate gave some fractionation of the antibody population.

References (26)

  • J.E. Bata et al.

    Immunochemistry

    (1964)
  • H. Weigel

    Adv. Carbohyd. Chem.

    (1963)
  • E.A. Kabat et al.

    Am. J. Med.

    (1948)
  • B.B.L. Agrawal et al.

    Biochim. Biophys. Acta

    (1967)
  • S.G. Nathenson et al.

    J.biol. Chem.

    (1962)
  • S.G. Nathenson et al.

    J. biol. Chem.

    (1966)
  • P. Grabar et al.

    Biochim. biophys. Acta

    (1953)
  • L.L. So et al.

    Biochim. biophys. Acta

    (1968)
  • E.A. Kabat et al.

    Experimental Immunochemistry

    (1961)
  • W.B. Danliker et al.

    Biochemistry

    (1967)
  • S. Avrameas et al.

    Biochem. J.

    (1967)
  • Y. Arakatsu et al.

    J. Immun.

    (1966)
  • S. Hammaström et al.

    Biochemistry

    (1969)
  • Cited by (16)

    View all citing articles on Scopus

    Aided by grants from the National Sciene Foundation (GB-8341), the Office of Naval Research (Nonr 266(13)) and a General Research Support Grant from the United States Public Health Service.

    Fellowship supported by the American Scandinavian Foundation and by AB Pharamcia, Uppsala, Sweden.

    View full text