Elsevier

Developmental Biology

Volume 72, Issue 2, October 1979, Pages 350-363
Developmental Biology

Full paper
Analysis of Xenopus laevis globins during development and erythroid cell maturation and the construction of recombinant plasmids containing sequences derived from adult globin mRNA

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Abstract

Developmental changes in the globin polypeptide composition of Xenopus laevis erythrocytes were examined by acid-urea polyacrylamide gel electrophoresis and a major switch from tadpole to adult globins was detected after metamorphic climax. The coding capacity of mRNA derived from mature adult erythroid cells was examined by cell-free translation and the products were shown to coelectrophorese with the globins of the adult erythroid cells. We describe the molecular cloning of sequences present in this mRNA and the characterisation of clones derived from one of the major globins and one clone derived from a minor adult globin mRNA.

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    Present address: Institut fur Molekularbiologie II der Universitat Zurich, 8057 Zurich, Switzerland.

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