Elsevier

Developmental Biology

Volume 2, Issue 3, June 1960, Pages 285-297
Developmental Biology

Embryonic potency of embryoid bodies derived from a transplantable testicular teratoma of the mouse

https://doi.org/10.1016/0012-1606(60)90010-5Get rights and content

Abstract

Testicular teratomas occur relatively frequently in inbred strain 129 mice. Occasionally they grow progressively and survive serial transplantation indefinitely. One such tumor has been maintained for approximately six years, and it still retains its highly pleomorphic nature. When established as an ascitic tumor, this teratoma is capable of producing thousands of free-floating formations that resemble 5- and 6-day mouse embryos. The aim of this investigation was to test the histogenetic capacities of these embryoid bodies. Single embryoid bodies were transplanted into the anterior chamber of the eyes of mature strain 129 mice. Growths resulting from these intraocular grafts were examined histologically and some were retransplanted subcutaneously to form solid tumor sublines. Both the intraocular and subcutaneous grafts contained many types of tissues, including a variety of epithelia; neural tissue; cartilage with peripheral ossification; smooth, cardiac, and voluntary muscle; trophoblastic giant cells; layers of ectoderm and endoderm; and undifferentiated embryonal cells. It is concluded that the embryoid bodies derived from the testicular teratomas of strain 129 mice have similarities in embryonic potency as well as in morphology to normal mouse embryos.

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This investigation was supported by research grant C-2662 from the National Cancer Institute, National Institutes of Health, Public Health Service, and by a grant, E-121, from the American Cancer Society, Inc.

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