The malachite green micromethod for the determination of inorganic phosphate
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Cited by (48)
Improving the stability of the malachite green method for the determination of phosphate using Pluronic F68
2020, Analytical BiochemistryCitation Excerpt :As mentioned, the addition of a surfactant is needed to avoid the formation of an insoluble dye. It has been reported that Tween 20, polyvinyl alcohol and Ultrawet 60 are good options for stabilizing the dye during the analytical procedure but not for long term storage of the color reagent [14–18]. In fact, Figure S5 shows the progressive decrease in sensitivity when the color reagent was prepared using Tween 20 as stabilizer [14].
Long-term protective effect of surface sealants against erosive wear by intrinsic and extrinsic acids
2012, Journal of DentistryCitation Excerpt :The amount of enamel was quantified by assignation of 32P in the collected solutions by determining the Cherenkow radiation and comparing this radiation with the radiation of known amounts of apatite. Different other studies concerning prevention of erosive dental hard tissue loss have also measured the amount of certain apatite minerals in the demineralisation solution by chemical analysis of minerals dissolved in the used erosive agent by Arsenazo III procedure,42,43 atomic absorption spectroscopy44 or by colorimetric methods.45,46 As one of the used surface sealants (Silicon Seal Nano Mix) contains apatite, it was not feasible to detect certain apatite minerals in the used solutions since the above listed methods are not able to differentiate between minerals dissolved from the sealed enamel or from the used surface sealant.
Foot-and-mouth disease virus 2C is a hexameric AAA+ protein with a coordinated ATP hydrolysis mechanism
2010, Journal of Biological ChemistryCitation Excerpt :Except where stated otherwise, the soluble 2C proteins obtained were concentrated in the gel filtration buffer to 20 mg/ml and stored at −80 °C. ATP hydrolysis was measured with a colorimetric assay based on the malachite green dye method described previously (50, 51). ATPase assays were performed in 96-well plates in 100-μl reaction volumes containing 50 mm HEPES, pH 7.3, 1 mm dithiothreitol, 2 mm MgCl2 in the presence of 2C (typically 1.25 μm) and ATP (or another NTP) at the concentrations indicated in the relevant figure legends.
Ecto-nucleoside triphosphate diphosphohydrolase 1 (E-NTPDase1/CD39) regulates neutrophil chemotaxis by hydrolyzing released ATP to adenosine
2008, Journal of Biological ChemistryCitation Excerpt :Nucleotides were identified, and their concentrations were estimated using their retention times as compared with known standards. Malachite Green Assay—Solutions of malachite green (0.812 g/liter deionized H2O), ammonium molybdate (0.3 m in 6 n HCl), and polyvinyl alcohol (23.2 g/liter H2O) were prepared as described previously (14). To produce the assay solution, 4 ml of malachite green solution was mixed with 2 ml each of the ammonium molybdate and polyvinyl alcohol solutions.
Surfactant-sensitized malachite green method for trace determination of orthophosphate in aqueous solution
2006, Analytica Chimica ActaCitation Excerpt :Although there are no direct measurements on the extent of hydrolysis of the organic phosphorus compounds by the MG method, it is likely that high concentrations of both acid and molybdate used in MG method can induce the hydrolysis of acid-labile organic phosphorus compounds, such as simple phosphorus sugars and monophosphate esters. This might explain the results from the inter-comparison study that the orthophosphate concentrations in natural waters and soil extracts measured by malachite green method are systematically higher than that by molybdenum blue method [30,31]. The ion association of phosphomolybdate with malachite green (MG) is a slow process, and the absorbance of colored product can continue to increase for many hours [11].